[1]焦凯瑞,邓 波,宋 萍,等.浓香型白酒生产中的功能微生物及其协同作用研究进展[J].南京师大学报(自然科学版),2024,(04):68-76.[doi:10.3969/j.issn.1001-4616.2024.04.008]
 Jiao Kairui,Deng Bo,Song Ping,et al.Research Progress of Functional Microorganisms and Their Synergistic Effects in Luzhou-flavor Liquor Production[J].Journal of Nanjing Normal University(Natural Science Edition),2024,(04):68-76.[doi:10.3969/j.issn.1001-4616.2024.04.008]
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浓香型白酒生产中的功能微生物及其协同作用研究进展()
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《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

卷:
期数:
2024年04期
页码:
68-76
栏目:
生物学
出版日期:
2024-12-15

文章信息/Info

Title:
Research Progress of Functional Microorganisms and Their Synergistic Effects in Luzhou-flavor Liquor Production
文章编号:
1001-4616(2024)04-0068-09
作者:
焦凯瑞1邓 波2宋 萍3连 宾4
(1.南京师范大学生命科学学院,江苏 南京 210023)
(2.国家固态酿造工程技术研究中心,四川 泸州 646000)
(3.南京师范大学食品与制药工程学院,江苏 南京 210023)
(4.南京师范大学海洋科学与工程学院,江苏 南京 210023)
Author(s):
Jiao Kairui1Deng Bo2Song Ping3Lian Bin4
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)
(2.National Engineering Research Center of Solid State Brewing,Luzhou 646000,China)
(3.School of Food Science and Pharmaceutical Engineering,Nanjing Normal University,Nanjing 210023,China)
(4.College of Marine Science and Engineering,Nanjing Normal University,Nanjing 210023,China)
关键词:
浓香型白酒窖泥酒醅大曲微生物协同作用
Keywords:
Luzhou-flavor liquorpit mudfermented grainsDaqumicrobial synergy
分类号:
TS201.3
DOI:
10.3969/j.issn.1001-4616.2024.04.008
文献标志码:
A
摘要:
浓香型白酒因其窖香浓郁、香味协调,在中国白酒市场最为畅销. 它是以谷物为原料,采用中温大曲作为糖化发酵剂,经过固态发酵、蒸馏、陈酿、勾调而成的发酵食品. 在浓香型白酒酿造过程中涉及大曲、窖泥和酒醅中多种类型微生物的参与,不同的酿酒微生物在不同的发酵阶段协同驱动发酵原料发生一系列复杂的生物化学转化,并最终影响白酒的风味特征和品质. 基于现有的研究报道,本文从浓香型白酒大曲、酒醅和窖泥中的优势微生物类群、优势微生物功能、微生物之间的协同作用及其对白酒风味的影响等方面综述了浓香型白酒功能微生物的研究进展,并展望了白酒微生物的未来研究方向,为浓香型白酒的品质提升提供基础资料.
Abstract:
Luzhou-flavor liquor is the best-selling liquor in the Chinese liquor market because of its strong cellar aroma and harmonious fragrance. It is a fermented food made from grains,using medium-temperature Daqu as the saccharification and fermentation agent,and undergoing solid-state fermentation,distillation,aging and blending. The brewing process of Luzhou-flavor liquor involves the participation of multiple types of microorganisms in the Daqu,pit mud,and fermented grains,and different brewing microorganisms synergistically drive a series of complex biochemical transformations of fermented raw materials at different stages of fermentation,which ultimately affects the flavor characteristics and quality of liquor. Based on the existing research reports,this paper summarizes the research progress of functional microorganisms in Luzhou-flavor liquor from the dominant microbial taxa in the Daqu,fermented grains and pit mud,the functions of the dominant microorganisms,the synergistic effects among varied microorganisms and their influence on the flavor of the liquor,etc.,and proposes an outlook of the future direction of related functional microorganisms,in order to provide the basic information for the improvement of the quality of Luzhou-flavor liquor.

参考文献/References:

[1]LIU H L,SUN B G. Effect of fermentation processing on the flavor of Baijiu[J]. Journal of agricultural and food chemistry,2018,66(22):5425-5432.
[2]胡景辉,陈禹锜,薛新新,等. 浓香型白酒发展概述[J]. 中国酿造,2022,41(6):24-30.
[3]JIN G Y,ZHU Y,XU Y. Mystery behind chinese liquor fermentation[J]. Trends in food science & technology,2017,63:18-28.
[4]ZOU W,ZHAO C Q,LUO H B. Diversity and function of microbial community in Chinese strong-flavor Baijiu ecosystem:a review[J]. Frontiers in microbiology,2018,9:671.
[5]程伟,吴丽华,徐亚磊,等. 浓香型白酒酿造微生物研究进展[J]. 中国酿造,2014,33(3):1-4.
[6]LIU M K,TANG Y M,GUO X J,et al. Structural and functional changes in prokaryotic communities in artificial pit mud during Chinese Baijiu production[J]. Msystems,2020,5(2):e00829-19.
[7]FU J X,CHEN L,YANG S Z,et al. Metagenome and analysis of metabolic potential of the microbial community in pit mud used for Chinese strong-flavor liquor production[J]. Food research international,2021,143:110294.
[8]MU Y,HUANG J,ZHOU R Q,et al. Effects of daqu attributes on distribution and assembly patterns of microbial communities and their metabolic function of artificial pit mud[J]. Foods,2022,11(18):2922.
[9]HE G Q,HUANG J,ZHOU R Q,et al. Effect of fortified daqu on the microbial community and flavor in Chinese strong-flavor liquor brewing process[J]. Frontiers in microbiology,2019,10:56.
[10]WEI Y,ZOU W,SHEN C H,et al. Basic flavor types and component characteristics of Chinese traditional liquors:a review[J]. Journal of food science,2020,85(12):4096-4107.
[11]高江婧,任聪,刘国英,等. 浓香型白酒酒醅风味物质和微生物菌群结构随发酵时间的变化规律[J]. 食品与发酵工业,2019,45(20):1-6.
[12]李俊刚,郭文宇,罗英,等. 利用GC-MS法对不同窖龄下浓香型白酒风味物质的研究[J]. 中国酿造,2015,34(9):141-144.
[13]WANG H L,GU Y,ZHOU W C,et al. Adaptability of a caproate-producing bacterium contributes to its dominance in an anaerobic fermentation system[J]. Applied and environmental microbiology,2021,87(20):e120321.
[14]ZHAO Q W,ZHU H W,TONG X,et al. Comparative genomic analyses reveal carbohydrates-rich environment adaptability of Lentilactobacillus laojiaonis sp. Nov. Im3328[J]. Food bioscience,2023,53:102737.
[15]ZHANG Z D,DONG Y,XIANG F S,et al. Analysis of bacterial diversity and genetic evolution of Lacticaseibacillus paracasei isolates in fermentation pit mud[J]. Journal of applied microbiology,2022,133(3):1821-1831.
[16]HE G Q,HUANG J,WU C D,et al. Bioturbation effect of fortified daqu on microbial community and flavor metabolite in Chinese strong-flavor liquor brewing microecosystem[J]. Food research international,2020,129:108851.
[17]MU Y,HUANG J,ZHOU R Q,et al. Exploring the response patterns of strong-flavor Baijiu brewing microecosystem to fortified daqu under different pit ages[J]. Food research international,2022,155:111062.
[18]邓杰,黄治国,卫春会,等. 基于高通量测序的浓香型白酒窖池细菌群落结构分析[J]. 现代食品科技,2015,31(7):50-55.
[19]YANG Y,WANG S T,LU Z M,et al. Metagenomics unveils microbial roles involved in metabolic network of flavor development in medium-temperature daqu starter[J]. Food research international,2021,140:110037.
[20]LIU M K,LIU C Y,TIAN X H,et al. Bioremediation of degraded pit mud by indigenous microbes for Baijiu production[J]. Food microbiology,2022,108:104096.
[21]CHAI L J,QIAN W,ZHONG X Z,et al. Mining the factors driving the evolution of the pit mud microbiome under the impact of long-term production of strong-flavor Baijiu[J]. Applied and environmental microbiology,2021,87(17):e88521.
[22]LIU J,WU Q,WANG P,et al. Synergistic effect in core microbiota associated with sulfur metabolism in spontaneous Chinese liquor fermentation[J]. Applied and environmental microbiology,2017,83(24):e01475-17.
[23]ZHOU W,LIAO Z M,WU Z Y,et al. Analysis of the difference between aged and degenerated pit mud microbiome in fermentation cellars for Chinese Luzhou-flavor Baijiu by metatranscriptomics[J]. Journal of the science of food and agriculture,2021,101(11):4621-4631.
[24]GUAN T W,YANG H,OU M Y,et al. Storage period affecting dynamic succession of microbiota and quality changes of strong-flavor baijiu daqu[J]. LWT-food science and technology,2021,139:110544.
[25]黄平,王子豪,郑佳,等. 浓香型白酒大曲微生物群落结构研究进展[J]. 微生物学通报,2023,50(7):3170-3186.
[26]李兵,张超,王玉霞,等. 白酒大曲功能微生物与酶系研究进展[J]. 中国酿造,2019,38(6):7-12.
[27]SAKANDAR H A,HUSSAIN R,FARID K Q,et al. Functional microbiota in Chinese traditional Baijiu and mijiu qu(starters):a review[J]. Food research international,2020,138:109830.
[28]DU H,WANG X S,ZHANG Y H,et al. Exploring the impacts of raw materials and environments on the microbiota in Chinese daqu starter[J]. International journal of food microbiology,2019,297:32-40.
[29]XIAO C,LU Z M,ZHANG X J,et al. Bio-heat is a key environmental driver shaping the microbial community of medium-temperature Daqu[J]. Applied and environmental microbiology,2017,83(23):e01550-17.
[30]WANG X S,DU H,XU Y. Source tracking of prokaryotic communities in fermented grain of Chinese strong-flavor liquor[J]. International journal of food microbiology,2017,244:27-35.
[31]敖灵,梅军兰,郎召伟,等. 泸型酒酿造过程中各层酒醅挥发性物质变化规律[J]. 中国酿造,2022,41(5):28-33.
[32]汪慧慧,高俊杰,李庆腾,等. 浓香型酒醅发酵过程中真核微生物群落的研究[J]. 食品科技,2022,47(8):14-20.
[33]CHAI L J,LU Z M,ZHANG X J,et al. Zooming in on butyrate-producing clostridial consortia in the fermented grains of Baijiu via gene sequence-guided microbial isolation[J]. Frontiers in microbiology,2019,10:1397.
[34]XU S S,ZHANG M Z,XU B Y,et al. Microbial communities and flavor formation in the fermentation of Chinese strong-flavor Baijiu produced from old and new Zaopei[J]. Food research international,2022,156:111162.
[35]JIAO W J,XIE F,GAO L,et al. Identification of core microbiota in the fermented grains of a Chinese strong-flavor liquor from Sichuan[J]. LWT-food science and technology,2022,158:113140.
[36]LIU M K,TANG Y M,ZHAO K,et al. Determination of the fungal community of pit mud in fermentation cellars for Chinese strong-flavor liquor,using DGGE and Illumina MiSeq sequencing[J]. Food research international,2017,91:80-87.
[37]高占争,吴正云,张文学. 浓香型白酒窖泥微生物群落研究进展[J]. 中国酿造,2020,39(6):1-7.
[38]吴浪涛,丁文骏,谢专,等. 不同时期窖泥理化因子、风味物质和细菌组成的相关性[J]. 微生物学通报,2022,49(3):1030-1047.
[39]ZHANG H M,MENG Y J,WANG Y L,et al. Prokaryotic communities in multidimensional bottom-pit-mud from old and young pits used for the production of Chinese strong-flavor Baijiu[J]. Food chemistry,2020,312:126084.
[40]LIU M K,TANG Y M,GUO X J,et al. Deep sequencing reveals high bacterial diversity and phylogenetic novelty in pit mud from Luzhou Laojiao cellars for Chinese strong-flavor Baijiu[J]. Food research international,2017,102:68-76.
[41]张应刚,许涛,郑蕾,等. 窖泥群落结构及功能微生物研究进展[J]. 微生物学通报,2021,48(11):4327-4343.
[42]TAO Y,WANG X,LI X Z,et al. The functional potential and active populations of the pit mud microbiome for the production of Chinese strong-flavour liquor[J]. Microbial biotechnology,2017,10(6):1603-1615.
[43]CHAI L J,XU P X,QIAN W,et al. Profiling the Clostridia with butyrate-producing potential in the mud of Chinese liquor fermentation cellar[J]. International journal of food microbiology,2019,297:41-50.
[44]罗晶,祝水兰,王丽,等. 浓香型白酒酿造微生物与风味物质组成的研究进展[J]. 中国酿造,2020,39(4):1-6.
[45]陈继良. 浓香型白酒在酿造中的功能菌研究进展[J]. 酿酒,2020,47(2):15-18.
[46]胡晓龙,田瑞杰,李保坤,等. 基于宏转录组学技术解析浓香型酒醅活性微生物群落结构及功能变化特征[J]. 食品科学,2022,43(10):124-132.
[47]王莉,袁宁,管桂坤,等. 黄水对浓香型白酒酒质的影响[J]. 酿酒科技,2020(10):83-86.
[48]QIAN W,LU Z M,CHAI L J,et al. Cooperation within the microbial consortia of fermented grains and pit mud drives organic acid synthesis in strong-flavor Baijiu production[J]. Food research international,2021,147:110449.
[49]信春晖,许玲,董乔娟,等. 酿酒微生物对酒体质量及风格的影响[J]. 酿酒,2016,43(2):38-47.
[50]GAO J J,LIU G Y,LI A J,et al. Domination of pit mud microbes in the formation of diverse flavour compounds during Chinese strong aroma-type Baijiu fermentation[J]. LWT,2021,137:110442.
[51]ZOU W,YE G B,ZHANG K Z. Diversity,function,and application of Clostridium in Chinese strong flavor Baijiu ecosystem:a review[J]. Journal of food science,2018,83(5):1193-1199.
[52]PU S C,YAN S B. Fungal diversity profiles in pit mud samples from Chinese strong-flavour liquor pit[J]. Foods,2022,11(22):3544.
[53]JIAO K R,DENG B,SONG P,et al. Difference analysis of the composition of iron(Hydr)oxides and dissolved organic matter in pit mud of different pit ages in Luzhou Laojiao and its implications for the ripening process of pit mud[J]. Foods,2023,12(21):3962.
[54]GUAN T W,LIN Y J,CHEN K B,et al. Physicochemical factors affecting microbiota dynamics during traditional solid-state fermentation of Chinese strong-flavor Baijiu[J]. Frontiers in microbiology,2020,11:2090.

备注/Memo

备注/Memo:
收稿日期:2023-09-23.
基金项目:泸州老窖-南京师范大学文化与科技创新研究中心自主研究项目(CY2X202200201).
通讯作者:连宾,博士,教授,博士生导师,研究方向:资源与环境微生物,地质微生物. E-mail:lianbin@njnu.edu.cn; 宋萍,博士,副教授,研究方向:微生物天然产物生物合成与合成生物学. E-mail:songping@njnu.edu.cn
更新日期/Last Update: 2024-12-15