[1]胡梦臻,王聪聪,张池莹,等.南京市入侵福寿螺的物种鉴定与遗传多样性分析[J].南京师大学报(自然科学版),2026,49(02):67-73.[doi:10.3969/j.issn.1001-4616.2026.02.007]
 Hu Mengzhen,Wang Congcong,Zhang Chiying,et al.Species Identification and Genetic Diversity of Invasive Pomacea spp. in Nanjing City[J].Journal of Nanjing Normal University(Natural Science Edition),2026,49(02):67-73.[doi:10.3969/j.issn.1001-4616.2026.02.007]
点击复制

南京市入侵福寿螺的物种鉴定与遗传多样性分析()

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

卷:
49
期数:
2026年02期
页码:
67-73
栏目:
生态学
出版日期:
2026-04-10

文章信息/Info

Title:
Species Identification and Genetic Diversity of Invasive Pomacea spp. in Nanjing City
文章编号:
1001-4616(2026)02-0067-07
作者:
胡梦臻1王聪聪1张池莹2黄 映1肖溢洁2储海燕1李 宏1陈 炼2
1.南京师范大学生命科学学院,江苏 南京 210023
2.南京林业大学生命科学学院,江苏 南京 210037
Author(s):
Hu Mengzhen1Wang Congcong1Zhang Chiying2Huang Ying1Xiao Yijie2Chu Haiyan1Li Hong1Chen Lian2
1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
2.College of Life Sciences,Nanjing Forestry University,Nanjing 210037,China
关键词:
生物入侵福寿螺线粒体COI基因杂交遗传多样性
Keywords:
biological invasion Pomacea mtDNA COI gene hybridization genetic diversity
分类号:
Q958.1
DOI:
10.3969/j.issn.1001-4616.2026.02.007
文献标志码:
A
摘要:
福寿螺属(Pomacea)包含多个物种,其中小管福寿螺与斑点福寿螺形态近似,入侵能力较强. 为明确南京市福寿螺种类及遗传多样性,本研究基于线粒体细胞色素氧化酶亚基 I(COI)基因序列和核基因EF1α分型方法,对采自南京市9个区的河流及公园的114只福寿螺及34块卵块样本进行分析. 经线粒体COI基因序列比对,南京市入侵福寿螺包括小管福寿螺和斑点福寿螺,其中小管福寿螺为入侵南京的优势种,未发现隐秘福寿螺. 核基因EF1α分型分析表明,南京市福寿螺中已出现小管福寿螺和斑点福寿螺的杂交个体,占比24.56%. 本研究结果为南京地区福寿螺的入侵风险评估与科学防治提供了基础数据和背景资料.
Abstract:
The genus Pomacea includes multiple species, with Pomacea canaliculata and P.maculata being morphologically similar and highly invasive. To identify Pomacea species and assess their genetic diversity in Nanjing City, the study analyzed 114 adult snails and 34 egg mass samples collected from rivers and park waters across 9 districts based on mitochondrial cytochrome c oxidase subunit I(COI)gene sequences and nuclear EF1α genotyping. All samples collected in Nanjing were identified as either P.canaliculata or P.maculata, with P.canaliculata being the predominant species. No occurrence of P.occulata was detected. Nuclear gene EF1α genotyping revealed hybrid individuals between P.canaliculata and P.maculata in Nanjing, which constituted 24.56% of the total sampled individuals. These findings provide essential baseline data for risk assessment and targeted management strategies against Pomacea invasions in Nanjing.

参考文献/References:

[1]Cowie R H,Hayes K A,Thiengo S C. Global advances in ecology and management of golden apple snails[M]. Science City of Muñoz,Nueva Ecija:Philippine Rice Research Institute,2006.
[2]钱子衿,林友福,杨雨婷,等. 苏州市入侵福寿螺的遗传多样性[J]. 动物学杂志,2021,56(6):929-938.
[3]Brito F C,Joshi R C. The golden apple snail Pomacea canaliculata:a review on invasion,dispersion and control[J]. Outlooks on Pest Management,2016,27(4):157-163.
[4]Matsukura K,Okuda M,Cazzaniga N J,et al. Genetic exchange between two freshwater apple snails,and invading East and Southeast Asia[J]. Biological Invasions,2013,15(9):2039-2048.
[5]Wei R,Chang Y W,Xie H F,et al. Population genetic structure of Pomacea canaliculata in China based on the COI and ITS1 genes[J]. Scientific Reports,2024,14(1):12045.
[6]Yang Q Q,Liu S W,He C,et al. Distribution and the origin of invasive apple snails,Pomacea canaliculata and P.maculata(Gastropoda:Ampullariidae)in China[J]. Scientific Reports,2018,8(1):1185.
[7]Lowe S,Browne M,Boudjelas S,et al. 100 of the world's worst invasive alien species:a selection from the global invasive species database[M]. Auckland:Invasive Species Specialist Group,2000.
[8]魏然,吴承东,谢洪芳,等. 入侵性有害生物福寿螺在江苏的风险分析[J]. 扬州大学学报(农业与生命科学版),2021,42(6):119-124,137.
[9]Karraker N E,Dudgeon D. Invasive apple snails(Pomacea canaliculata)are predators of amphibians in South China[J]. Biological Invasions,2014,16(9):1785-1789.
[10]张佳佳. 浅谈稻田福寿螺绿色防控技术与运用[J]. 江西农业,2025(7):99-101.
[11]Sin T S. Evaluation of different species of fish for biological control of golden apple snail Pomacea canaliculata(Lamarck)in rice[J]. Crop Protection,2006,25(9):1004-1012.
[12]Liu M Y,Sui C R,Zhao W Y,et al. Dynamic response of gut microbiota mediates the adaptation of Cipangopaludina chinensis to Pomacea canaliculata invasion[J]. Microbiome,2025,13(1):171.
[13]Kwong K L,Dudgeon D,Wong P K,et al. Secondary production and diet of an invasive snail in freshwater wetlands:implications for resource utilization and competition[J]. Biological Invasions,2010,12(5):1153-1164.
[14]Hayes K A,Cowie R H,Thiengo S C,et al. Comparing apples with apples:clarifying the identities of two highly invasive Neotropical Ampullariidae(Caenogastropoda)[J]. Zoological Journal of the Linnean Society,2012,166(4):723-753.
[15]Yang Q Q,He C,Liu G F,et al. Introgressive hybridization between two non-native apple snails in China:widespread hybridization and homogenization in egg morphology[J]. Pest Management Science,2020,76(12):4231-4239.
[16]李迦南,赵星星,王恩,等. 西湖水域外来生物福寿螺的种类鉴定及种群遗传多样性研究[J]. 水生生物学报,2022,46(3):355-363.
[17]Matsukura K,Izumi Y,Yoshida K,et al. Cold tolerance of invasive freshwater snails,Pomacea canaliculata,P.maculata,and their hybrids helps explain their different distributions [J]. Freshwater Biology,2016,61(1):80-87.
[18]蒲佳佳,杨平俊,戴洋,等. 长江下游外来生物福寿螺的种类及其种群遗传结构[J]. 生物多样性,2023,31(3):117-130.
[19]Hayes K A,Joshi R C,Thiengo S C,et al. Out of South America:multiple origins of non-native apple snails in Asia[J]. Diversity and Distributions,2008,14(4):701-712.
[20]Tamura K,Stecher G,Kumar S. MEGA 11:molecular evolutionary genetics analysis version 11[J]. Molecular Biology and Evolution,2021,38(7):3022-3027.
[21]Rozas J S,Sánchez-DelBarrio J C,Messeguer X,et al. DnaSP,DNA polymorphism analyses by the coalescent and other methods[J]. Bioinformatics,2003,19(18):2496-2497.
[22]Posada D,Buckley T R. Model selection and model averaging in phylogenetics:advantages of akaike information criterion and bayesian approaches over likelihood ratio tests[J]. Systematic Biology,2004,53(5):793-808.
[23]Busschau T,Conradie W,Daniels S R. One species hides many:Molecular and morphological evidence for cryptic speciation in a thread snake(Leptotyphlopidae:Leptotyphlops sylvicolus Broadley & Wallach,1997)[J]. Journal of Zoological Systematics and Evolutionary Research,2021,59(1):195-221.
[24]Letunic I,Bork P. Interactive Tree Of Life(iTOL)v4:recent updates and new developments[J]. Nucleic Acids Research,2019,47(W1):W256-W259.
[25]Lv S,Zhang Y,Liu H X,et al. Phylogenetic evidence for multiple and secondary introductions of invasive snails:Pomacea species in the People's Republic of China[J]. Diversity and Distributions,2013,19(1):147-156.
[26]Matsukura K,Okuda M,Kubota K,et al. Genetic divergence of the genus Pomacea(Gastropoda:Ampullariidae)distributed in Japan,and a simple molecular method to distinguish P.canaliculata and P.insularum[J]. Applied Entomology and Zoology,2008,43(4):535-540.
[27]Yang Q Q,Liu S W,He C,et al. Invisible apple snail invasions:importance of continued vigilance and rigorous taxonomic assessments [J]. Pest Management Science,2019,75(5):1277-1286.
[28]Frankham R,Ballou J D,Briscoe D A. Introduction to conservation genetics[M]. UK:Cambridge University Press,2002.
[29]Yang Q Q,Ip J C H,Zhao X X,et al. Molecular analyses revealed three morphologically similar species of non-native apple snails and their patterns of distribution in freshwater wetlands of Hong Kong[J]. Diversity and Distributions,2022,28(1):97-111.
[30]吕山,张仪,刘和香,等. 中国大陆福寿螺种群遗传学研究[J]. 中国血吸虫病防治杂志,2011,23(2):178-182.
[31]王龙江,许艳,孙慧,等. 山东省首次发现福寿螺入侵[J]. 中国血吸虫病防治杂志,2022,34(4):407-411.
[32]陶刚,王华. 安徽省福寿螺入侵现状、扩散趋势及防治对策研究[J]. 农村科学实验,2025,12:166-168.
[33]Eyer P A,Blumenfeld A J,Johnson L N L,et al. Extensive human-mediated jump dispersal within and across the native and introduced ranges of the invasive termite Reticulitermes flavipes[J]. Molecular Ecology,2021,30(16):3948-3964.
[34]Jerde C L,Mahon A R,Chadderton W L,et al. "Sight-unseen" detection of rare aquatic species using environmental DNA[J]. Conservation Letters,2011,4(2):150-157.

备注/Memo

备注/Memo:
收稿日期:2025-10-30.
基金项目:国家自然科学基金项目(32170434).
通讯作者:陈炼,博士,教授,研究方向:入侵生物学. E-mail:chenlian_2004@163.com
更新日期/Last Update: 2026-04-10