[1]宋晓彬,范春娥,王 烨,等.小鼠脑微血管内皮细胞的分离培养与鉴定[J].南京师范大学学报(自然科学版),2017,40(03):128.[doi:10.3969/j.issn.1001-4616.2017.03.021]
 Song Xiaobin,Fan Chune,Wang Ye,et al.Primary Culture and Identification of Mouse BrainMicrovascular Endothelial Cell[J].Journal of Nanjing Normal University(Natural Science Edition),2017,40(03):128.[doi:10.3969/j.issn.1001-4616.2017.03.021]
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小鼠脑微血管内皮细胞的分离培养与鉴定()
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《南京师范大学学报》(自然科学版)[ISSN:1001-4616/CN:32-1239/N]

卷:
第40卷
期数:
2017年03期
页码:
128
栏目:
·生命科学·
出版日期:
2017-09-30

文章信息/Info

Title:
Primary Culture and Identification of Mouse BrainMicrovascular Endothelial Cell
文章编号:
1001-4616(2017)03-0138-06
作者:
宋晓彬1范春娥1王 烨2张艺腾1崔丛丛1曾卫卫1郑丽蓉1蔡晓洁1陈华群1
(1.南京师范大学生命科学学院,江苏省分子医学生物技术重点实验室,江苏 南京 210023)(2.南京沐赛生物技术有限公司,江苏 南京 210061)
Author(s):
Song Xiaobin1Fan Chun’e1Wang Ye2Zhang Yiteng1Cui Congcong1Zeng Weiwei1Zheng Lirong1Cai Xiaojie1Chen Huaqun1
(1.School of Life Sciences,Nanjing Normal University,Jiangsu Key Laboratory for Molecular and Medical Biotechnology,Nanjing 2l0023,China)(2.Nanjing Mucyte Biotechnical Com.,Nanjing 210061,China)
关键词:
脑微血管内皮细胞原代培养密度梯度离心酶消化
Keywords:
mouse brain microvescular endothelial cellprimary culturedensity gradient centrifugationenzyme digestion
分类号:
Q28
DOI:
10.3969/j.issn.1001-4616.2017.03.021
文献标志码:
A
摘要:
脑微血管内皮细胞(Brain microvascular endothelial cell,BMVEC)是构成血脑屏障(Brain-blood barrier,BBB)的基本组分,多种神经疾病都与BBB的失功能相关,分离培养原代BMVEC是体外研究BBB功能及其调节的重要手段. 本文通过简单有效的葡聚糖密度梯度离心和酶消化方法,从小鼠脑组织分离培养出BMVEC,并用免疫荧光染色进行了鉴定. 结果表明,内皮细胞表面特征性的标记分子CD31阳性细胞达到95%以上,同时通过VE-Cadherin及ZO-1免疫荧光染色鉴定证实,其为脑微血管内皮细胞. 本研究为体外利用BMVEC进行相关研究提供了良好基础.
Abstract:
Brain microvascular endothelial cell(BMVEC)is an essential component of the brain blood barrier(BBB). It has been known that the dysfunction of BBB plays critical roles in the pathological process of a variety of neurological disorders. The primary cultured BMVECs were widely used in the study of the functions and the regulation mechanisms of the BBB. Here,it is reported that a simple and effective method of isolating and growing of BMVEC established upon density gradient centrifugation and enzyme digestion. The purity of the cultured cells was higher than 95% determined by CD31 immunofluorescence staining. The features of the cells were further confirmed by positive staining of VE-cadherin and ZO-1,the characteristics of BMVECs. Our method may provide a good approach for the study of BBB in vitro.

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备注/Memo

备注/Memo:
收稿日期:2017-04-18.
基金项目:国家自然科学基金(31371356、30971540)、江苏省教育厅重大项目(13KJA180004).
通讯联系人:陈华群,教授,研究方向:细胞生物学. E-mail:chenhuaqun@njnu.edu.cn
更新日期/Last Update: 2017-09-30