[1]李海梅,王滢莹,高弢,等.FgPDK1调控禾谷镰孢菌响应渗透胁迫研究[J].南京师范大学学报(自然科学版),2018,41(01):76.[doi:10.3969/j.issn.1001-4616.2018.01.014]
　Li Haimei,Wang Yingying,Gao Tao,et al.FgPDK1 Modulates Osmotic Stress Responses in Fusarium graminearum[J].Journal of Nanjing Normal University(Natural Science Edition),2018,41(01):76.[doi:10.3969/j.issn.1001-4616.2018.01.014]
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FgPDK1调控禾谷镰孢菌响应渗透胁迫研究()
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《南京师范大学学报》（自然科学版）[ISSN:1001-4616/CN:32-1239/N]

卷:
第41卷
期数:
2018年01期
页码:
76
栏目:
·生命科学·
出版日期:
2018-03-31

文章信息/Info

Title:
FgPDK1 Modulates Osmotic Stress Responses in Fusarium graminearum
文章编号:
1001-4616(2018)01-0076-07
作者:
李海梅1; 王滢莹1; 高弢2; 徐寸发3; 石志琦2; 陈健2; 许晓风1
(1.南京师范大学生命科学学院,江苏 南京 210023)(2.江苏省农业科学院农产品质量安全与营养研究所,江苏 南京 210014)(3.江苏省农业科学院中心实验室,江苏 南京 210014)
Author(s):
Li Haimei1; Wang Yingying1; Gao Tao2; Xu Cunfa3; Shi Zhiqi2; Chen Jian2; Xu Xiaofeng1
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)(2.Institute of Food Safety and Nutrition,Jiangsu Academy of Agriculture Science,Nanjing 210014,China)(3. Central Laboratory,Jiangsu Academy of Agriculture Science,Nanjing 210014,
关键词:
禾谷镰孢菌; 丙酮酸脱氢酶激酶; 渗透胁迫; 甘油; 细胞死亡
Keywords:
Fusarium graminearum; PDK; osmotic stress; glycerol; cell death
分类号:
S432.1
DOI:
10.3969/j.issn.1001-4616.2018.01.014
文献标志码:
A
摘要:
真菌为了应对渗透胁迫进化出包括高渗甘油途径(high osmolarity glycerol,HOG)在内的一系列调控网络,但其上游信号调控因子尚不明确. 本文以禾谷镰孢菌(Fusarium graminearum)为研究对象,在盐胁迫下通过遗传学和生理生化手段,研究了一个关键的线粒体能量代谢酶基因丙酮酸脱氢酶激酶(FgPDK1)在调控渗透胁迫方面的重要作用. 结果显示,(1)菌丝生长测定结果表明FgPDK1敲除突变体(ΔFgPDK1)对盐胁迫的敏感性较野生型(PH-1)显著增加;(2)与PH-1相比较,
Abstract:
Fungi’s HOG(high osmolarity glycerol)pathway in response to osmotic stress has been revealed. However,the upstream signaling regulator of the pathway remains unclear. In this study,we investigated the pivotal role of a mitochondrial energy metabolic gene encoding for pyruvate dehydrogenase kinase(FgPDK1)in Fusarium graminearum in the regulation of NaCl stress by using genetic and physiological-biochemical approaches. The main results have been obtained as follows,(1)Determination of hyphal growth indicated that ΔFgPDK1(an FgPDK1 knock-out mutant)was more sensitive than wild type(PH-1).(2)Compared to PH-1,NaCl stress induced more electrolyte leakage and higher accumulation of glycerol in the hyphae of ΔFgPDK1.(3)ROS accumulation,MDA content,and cell death increased significantly in ΔFgPDK1 as compared to PH-1 under NaCl stress.(4)The relative expression levels of anti-oxidative genes(SOD and CAT)were significantly lower in ΔFgPDK1 as compared to PH-1 under NaCl stress.(5)All of the above physiological and biochemical responses could be recovered to the level of PH-1 in ΔFgPDK1-C(a gain-of-function complementary strain of ΔFgPDK1). These results suggested that FgPDK1 played important roles in the regulation of HOG downstream pathway and the maintenance of anti-oxidative capacity in F.graminearum under high osmatic stress. This study provides new evidence for understanding the molecular mechanism in fungi’s osmatic stress adaption.

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相似文献/References:

[1]林玲,史建荣,陈怀谷,等.禾谷镰孢菌原生质体的制备和再生研究[J].南京师范大学学报(自然科学版),2001,24(01):88.
　Lin Ling,Shi Jianrong,Chen Huaigu,et al.Preparation and Regeneration of Proplasts for Fusarium garaminearum Schwabe[J].Journal of Nanjing Normal University(Natural Science Edition),2001,24(01):88.

备注/Memo

备注/Memo:
收稿日期:2017-11-02.
基金项目:国家重点研发计划专项(2017YFD0201105).
通讯联系人:许晓风,教授,博导,研究方向:生理生化调控以及分子遗传学. E-mail:xuxiaofeng@njnu.edu.cn
更新日期/Last Update: 2018-03-31