[1]韦光绪,唐 毅,殷志敏.GGT酶法合成γ-谷氨酰半胱氨酸[J].南京师范大学学报(自然科学版),2018,41(02):78.[doi:10.3969/j.issn.1001-4616.2018.02.014]
　Wei Guangxu,Tang Yi,Yin Zhimin.Enzymatic Synthesis of γ-Glutamyl Cysteine with GGT[J].Journal of Nanjing Normal University(Natural Science Edition),2018,41(02):78.[doi:10.3969/j.issn.1001-4616.2018.02.014]
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GGT酶法合成γ-谷氨酰半胱氨酸()
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《南京师范大学学报》（自然科学版）[ISSN:1001-4616/CN:32-1239/N]

卷:
第41卷
期数:
2018年02期
页码:
78
栏目:
·生命科学·
出版日期:
2018-06-30

文章信息/Info

Title:
Enzymatic Synthesis of γ-Glutamyl Cysteine with GGT
文章编号:
1001-4616(2018)02-0078-05
作者:
韦光绪; 唐 毅; 殷志敏
南京师范大学生命科学学院,生物化学与生物制品研究所,江苏省分子与医学生物技术重点实验室,江苏 南京 210023
Author(s):
Wei Guangxu; Tang Yi; Yin Zhimin
School of Life Sciences,Nanjing Normal University,Institute of Biochemistry and Biological Products,Jiangsu Key Laboratory for Molecular and Medical Biotechnology,Nanjing 210023,China
关键词:
γ-谷氨酰转肽酶; γ-谷氨酰半胱氨酸; 胱氨酸; 酶法合成
Keywords:
γ-glutamyl transpeptidase; γ-glutamylcysteine; cystine; enzymatic synthesis
分类号:
Q555.3
DOI:
10.3969/j.issn.1001-4616.2018.02.014
文献标志码:
A
摘要:
以大肠杆菌K-12基因组中克隆表达的γ-谷氨酰转肽酶作为催化剂,L-谷氨酰胺和胱氨酸为底物合成γ-谷氨酰胱氨酸,经还原生成γ-谷氨酰半胱氨酸.考察反应时间、酶浓度、补料方式等可能对酶促反应过程产生影响的因素,在L-谷氨酰胺0.1 mol/L、胱氨酸0.2 mol/L、酶浓度0.024 U/mL及pH为10.0的条件下,40 ℃反应12 h,γ-谷氨酰半胱氨酸的最大浓度为6.13 g/L,L-谷氨酰胺的最大转化率为24.5%.
Abstract:
Gamma-glutamyl transpeptidase(GGT),which was cloned from the genome of E.coli K-12,was used as a catalyst to synthesis γ-glutamylcystine,with L-glutamine and cystine as the substrate. After the reduction reaction and γ-glutamylcysteine was synthesized. We studied the effects of reaction time,initial enzyme concentration,feed mode of L-glutamine on the enzymatic process and find that the maximal production of GGC would be 6.13 g/L and the conversion rate of L-glutamine would be 24.5%,when the initial reaction conditions of 0.1 mol/L of L-glutamine,0.2 mol/L of cystine and 0.024 U/mL of GGT,and the reaction is carried out at pH 10.0 and 40 ℃ for 12 h.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2018-02-26.
基金项目:江苏省科技厅前瞻性研究项目(BY2013001-03).
通讯联系人:殷志敏,博士,教授,研究方向:生物化学及细胞生物学. E-mail:yinzhimin@njnu.edu.cn
更新日期/Last Update: 2018-11-06