[1]冯 梦,朱欣然,郑盈盈,等.血管紧张素Ⅱ诱导小鼠心肌肥大差异表达蛋白的鉴定与分析[J].南京师范大学学报(自然科学版),2018,41(03):78.[doi:10.3969/j.issn.1001-4616.2018.03.013]
　Feng Meng,Zhu Xinran,Zheng Yingying,et al.Identification and Analysis of Differentially Expressed Proteins inAngiotensin Ⅱ Induced-Mouse Model of Cardiac Hypertrophy[J].Journal of Nanjing Normal University(Natural Science Edition),2018,41(03):78.[doi:10.3969/j.issn.1001-4616.2018.03.013]
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血管紧张素Ⅱ诱导小鼠心肌肥大差异表达蛋白的鉴定与分析()
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《南京师范大学学报》（自然科学版）[ISSN:1001-4616/CN:32-1239/N]

卷:
第41卷
期数:
2018年03期
页码:
78
栏目:
·生命科学·
出版日期:
2018-09-30

文章信息/Info

Title:
Identification and Analysis of Differentially Expressed Proteins inAngiotensin Ⅱ Induced-Mouse Model of Cardiac Hypertrophy
文章编号:
1001-4616(2018)03-0078-07
作者:
冯 梦; 朱欣然; 郑盈盈; 张 朝; 张 茵
南京师范大学生命科学学院,江苏省分子医学生物技术重点实验室,江苏 南京 210023
Author(s):
Feng Meng; Zhu Xinran; Zheng Yingying; Zhang Zhao; Zhang Yin
School of Life Sciences,Nanjing Normal University,Jiangsu Key Laboratory for Molecular and Medical Biotechnology,Nanjing 210023,China
关键词:
心肌肥大; 蛋白质组学; 线粒体; 能量代谢
Keywords:
cardiac hypertrophy; proteomics; mitochondria; energy metabolism
分类号:
R331.3+1
DOI:
10.3969/j.issn.1001-4616.2018.03.013
文献标志码:
A
摘要:
采用蛋白质组学方法研究血管紧张素(Angiotensin Ⅱ,Ang Ⅱ)诱导小鼠心肌肥大模型中心脏蛋白差异表达的变化情况并探讨其病理生理学意义. 构建Ang Ⅱ诱导的小鼠心肌肥大模型,提取小鼠的心脏蛋白,采用双向电泳技术分离差异表达的蛋白,凝胶考染后切取差异蛋白点进行质谱检测(MALDI-TOF)分析,获取的数据采用Mascot软件在NCBInr数据库内检索. 结果发现Ang Ⅱ模型组小鼠心脏蛋白图谱中有34个蛋白点表达量与对照组有显著差异,质谱鉴定出14种差异表达的蛋白,其中高表达的蛋白有2个,低表达的蛋白有12个. Ang Ⅱ诱导小鼠心肌肥大模型中表达有差异的蛋白主要与能量代谢、氧化应激和细胞骨架有关.
Abstract:
To identify the differential expression of proteins in Angiotensin Ⅱ(Ang Ⅱ)induced-mouse model of cardiac hypertrophy and explore the pathophysiological significance. A mouse model of cardiac hypertrophy was established by subcutaneous administration of Ang Ⅱ with an osmotic pump-based controlled release system and then the cardiac proteins were extracted. The differentially expressed proteins were separated with two dimensional electrophoresis(2-DE)and analyzed by matrix assisted laser desorption ionization/time-of-flight mass spectrometry(MALDI-TOF-MS)and the original data were processed by Mascot then searched in NCBInr database. There were significant differences in the expression levels of 34 proteins between Ang Ⅱ and Control groups(P<0.05),14 differentially expressed proteins were identified according to the database,in which 12 were down-regulated and 2 were up-regulated. The differentially expressed proteins in Ang Ⅱ induced-mouse model of cardiac hypertrophy were mainly related to energy metabolism,oxidative stress and cytoskeleton.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2018-05-25.
基金项目:国家自然科学基金资助项目(30570662、30871228、31171302)、江苏省教育厅自然科学研究计划重大项目(16KJA18000)、江苏省优势学科资助项目(1164320H106).
通讯联系人:张茵,讲师,研究方向:心脏能量代谢. E-mail:zhangyin@njnu.edu.cn
更新日期/Last Update: 2018-11-19