[1]杨亚茹,茆少星,闫淑珍,等.两株植物内生菌促生和诱导辣椒植株对南方根结线虫抗性的比较分析[J].南京师范大学学报(自然科学版),2019,42(04):77-84.[doi:10.3969/j.issn.1001-4616.2019.04.011]
 Yang Yaru,Mao Shaoxing,Yan Shuzhen,et al.Comparative Analysis of Growth Promotion and Resistance Induction ofPepper Plants to Meloidogyne incognita by Two Endophytes[J].Journal of Nanjing Normal University(Natural Science Edition),2019,42(04):77-84.[doi:10.3969/j.issn.1001-4616.2019.04.011]
点击复制

两株植物内生菌促生和诱导辣椒植株对南方根结线虫抗性的比较分析()
分享到:

《南京师范大学学报》(自然科学版)[ISSN:1001-4616/CN:32-1239/N]

卷:
第42卷
期数:
2019年04期
页码:
77-84
栏目:
·生命科学·
出版日期:
2019-12-30

文章信息/Info

Title:
Comparative Analysis of Growth Promotion and Resistance Induction ofPepper Plants to Meloidogyne incognita by Two Endophytes
文章编号:
1001-4616(2019)04-0077-08
作者:
杨亚茹茆少星闫淑珍陆长梅
南师师范大学生命科学学院,江苏 南京 210023
Author(s):
Yang YaruMao ShaoxingYan ShuzhenLu Changmei
School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
关键词:
植物内生菌辣椒南方根结线虫促生抗性产量
Keywords:
endophyteCapsicum annuumMeloidogyne incognitagrowth promotionresistanceyield
分类号:
S43
DOI:
10.3969/j.issn.1001-4616.2019.04.011
文献标志码:
A
摘要:
本文以两株产ACC脱氨酶的内生菌蜡状芽孢杆菌SZ5和荧光假单胞菌DLJ1为材料,探讨其在辣椒促生和预防线虫病害方面的作用. 结果显示,虽然两菌剂处理不能促进种子萌发,但对幼苗生长有短暂促进作用; 促生作用消失后,植株体内次生代谢物质含量和抗氧化酶活性仍高于对照,DLJ1组高于SZ5组. 挑战接种南方根结线虫后,接菌组植物体内的POD等酶活性以及总黄酮等物质含量的提高幅度高于对照. 菌剂处理降低了植株染病率和染病程度,提高了辣椒产量和品质; 萌发期一次处理效果优于后期的多次处理,DLJ1的处理效果优于SZ5
Abstract:
Meloidogyne incognita is the most common soil borne disease of pepper. Two endophytes,SZ5(Bacillus cereus)and DLJ1(Pseudomonas fluorescens biovar I),which can produce ACC deaminase,were used as materials to study their effects on the growth and resistance of pepper plants to M.incognita. Results showed that although the two endophytes could not promote seed germination,they could promote seedling growth for a short time. After the short-term effect disappeared,the contents of secondary metabolites and the activities of antioxidant enzymes of plants in test groups were still higher than those in the control,and those in the DLJ1 group were higher than those in the SZ5 group. After challenge inoculation with M.incognita,the activities of POD etc. and the contents of total flavonoids in the test plants were higher than those in the control. Treatment with DLJ1 or SZ5 reduced the infection rate and degree of M.incognita in plants,increased the yield and quality of pepper fruits. The effects of once inoculation at germination stage were better than those of multiple inoculations at transplantation and later,those of DLJ1 inoculation was better than those of SZ5 inoculation. In a word,once DLJ1 inoculation at germination stage can effectively promote the growth of pepper plants,increase its resistance to M.incognita and increase the yield and quality of its fruits. These effects may be related to the effective colonization of strains at germination,the maintenance of antioxidant capacity and efficient response of plants to stress,as well as the effective reduction of ethylene production in plants at growth stage.

参考文献/References:

[1] PIEDRA B A,LOPEZPEREZ J A,BELLO A,et al. Screening of Cuarentino pepper(Capsicum annuum L.)for resistance to Meloidogyne incognita[J]. Nematropica,2006,36(1):13-24.
[2]VAN LOON L C,BAKKER P. Induced systemic resistance as a mechanism of disease suppression by rhizobacteria[M]//PGPR:Biocontrol and Biofertilization. Germany:Springer,2005.
[3]KLOEPPER J W,RYU C,ZHANG S. Induced systemic resistance and promotion of plant growth by Bacillus spp.[J]. Phytopathology,2004,94(11):1259-1266.
[4]SIDDIQUI I A,SHAUKAT S S. Systemic resistance in tomato induced by biocontrol bacteria against the root-knot nematode,Meloidogyne javanica is independent of salicylic acid production[J]. Journal of phytopathology,2004,152(1):48-54.
[5]COMPANT S,DUFFY B,NOWAK J,et al. Use of plant growth-promoting bacteria for biocontrol of plant diseases:principles,mechanisms of action,and future prospects[J]. Applied and environmental microbiology,2005,71(9):4951-4959.
[6]彭双,闫淑珍,陈双林. 具杀线虫活性植物内生细菌的筛选和活性产物[J]. 微生物学报,2011(3):368-376.
[7]云晶晶. 两株植物内生细菌诱导辣椒对南方根结线虫和辣椒疫霉抗性的机制分析[D]. 南京:南京师范大学,2017.
[8]PENROSE D M,GLICK B R. Levels of ACC and related compounds in exudates and extracts of canola seeds treated with ACC deaminase-containing plant growth-promoting bacteria[J]. Canadian journal of microbiology,2001,47:368-372.
[9]姚军朋,姚 拓,王小利. ACC脱氨酶的应用研究进展与评述[J]. 生物技术,2010(2):87-91.
[10]ZAHIR Z A,GHANI U,NAVEED M,et al. Comparative effectiveness of Pseudomonas and Serratia sp. containing ACC-deaminase for improving growth and yield of wheat(Triticum aestivum L.)under salt-stressed conditions[J]. Archives of microbiology,2009,191(5):415-424.
[11]付思娅,陈双林,闫淑珍. 植物内生细菌在辣椒体内的定殖动态及对辣椒疫病的防治效果[J]. 中国生物防治学报,2013(4):561-568.
[12]刘维红,闫淑珍,杨启银,等. ACC脱氨酶活性细菌筛选及其对番茄初生苗生长的影响[J]. 江苏农业科学,2006(2):80-84.
[13]孟莎莎,向太和,程慢成. 一种利用黄瓜苗繁殖根结线虫的方法[J]. 杭州师范大学学报(自然科学版),2011(1):56-58.
[14]彭双,杨茹,闫淑珍,等. 杀线虫植物内生细菌和根际放线菌对根结线虫的防效[J]. 植物保护学报,2012(1):63-69.
f[15]孙林华,杜建梅,孙 晴,等. 金花葵种子萌发特性及萌发期抗胁迫能力分析[J]. 种子,2014,33(11):5-9.
[16]刘彤彤,蒋欣梅,于锡宏,等. 间歇降温对黄瓜幼苗耐冷相关指标的影响[J]. 江苏农业科学,2016,44(12):195-196,271.
[17]CAKMAK I,MARSCHNER H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase,ascorbate peroxidase,and glutathione reductase in bean leaves[J]. Plant physiology,1992,98(4):1222-1227.
[18]刘太国,李永镐,陈万权. 水杨酸对感染 TMV 烟草叶片 PAL 活性的影响[J]. 西北农林科技大学学报(自然科学版),2006,33(B08):111-114.
[19]朱宏波,腾冰,高凤兰,等. 不同抗性大豆品种感染SMV_1后若干生化变化[J]. 西北农业学报,2001(3):38-40.
[20]王 静,睢 薇,滕 冰,等. 紫外-可见分光光度法定量测定沙棘果实中水溶性色素[J]. 中国林副特产,2001(2):2-3.
[21]滕 冰,吴宗璞. 大豆种粒中总多酚含量的分析方法[J]. 大豆科学,1999(3):265-268.
[22]GARABEDIAN S,VAN GUNDY S D. Use of avermectins for the control of Meloidogyne incognita on tomatoes[J]. Journal of nematology,1983,15(4):503.
[23]白春明,段玉玺,陈立杰,等 番茄品种对南方根结线虫的抗性评价[J]. 中国蔬菜,2010(6):33-37.
[24]赵荣秋,杨湘虹. 乙烯在种子休眠与萌发中的调控作用[J]. 长江大学学报(自然科学版),2016(33):47-51.
[25]HONTZEAS N,HONTZEAS C E,GLICK B R. Reaction mechanisms of the bacterial enzyme 1-aminocyclopropane-1-carboxylate deaminase[J]. Biotechnology advances,2006,24(4):420-426.
[26]李路,徐以华,梁梦琦,等. 水稻对穗枯病的抗病机理初步研究[J]. 中国水稻科学,2017,31(5):551-558.
[27]LEON J,LAWTON M A,RASKIN I. Hydrogen peroxide stimulates salicylic acid biosynthesis in tobacco[J]. Plant physiology,1995,108(4):1673-1678.
[28]JETIYANON K,KLOEPPER J W. Mixtures of plant growth-promoting rhizobacteria for induction of systemic resistance against multiple plant diseases[J]. Biological control,2002,24(3):285-291.
[29]GOTO D B,MIYAZAWA H,MAR J C,et al. Not to be suppressed?Rethinking the host response at a root-parasite interface[J]. Plant science,2013,213:9-17.

备注/Memo

备注/Memo:
收稿日期:2019-09-12.
基金项目:江苏省自然科学基金项目(BK2012848).
通讯联系人:陆长梅,副教授,研究方向:植物资源与保护. E-mail:08134@njnu.edu.cn
更新日期/Last Update: 2019-12-31