[1]李淑贤,林 英,祁 雯,等.斑点福寿螺肠道菌群结构及功能研究[J].南京师大学报(自然科学版),2022,(01):64-73.[doi:10.3969/j.issn.1001-4616.2022.01.010]
 Li Shuxian,Lin Ying,Qi Wen,et al.Research on the Bacterial Community Structure and Functions in the Intestine of Pomacea maculata[J].Journal of Nanjing Normal University(Natural Science Edition),2022,(01):64-73.[doi:10.3969/j.issn.1001-4616.2022.01.010]
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斑点福寿螺肠道菌群结构及功能研究()
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《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

卷:
期数:
2022年01期
页码:
64-73
栏目:
·生物学·
出版日期:
2022-03-15

文章信息/Info

Title:
Research on the Bacterial Community Structure and Functions in the Intestine of Pomacea maculata
文章编号:
1001-4616(2022)01-0064-10
作者:
李淑贤12林 英1祁 雯3陈 燏3李雪霞2朱善良3卫 蔚3李 宏1陈 炼2
(1.南京师范大学生命科学学院,江苏 南京 210023)(2.南京林业大学生物与环境学院,江苏 南京 210037)(3.江苏第二师范学院生命科学与化学学院,江苏 南京 210042)
Author(s):
Li Shuxian12Lin Ying1Qi Wen3Chen Yu3Li Xuexia2Zhu Shanliang3Wei Wei3Li Hong1Chen Lian2
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)(2.College of Biology and Environment,Nanjing Forestry University,Nanjing 210037,China)(3.School of Life Sciences and Chemistry,Jiangsu Second Normal University,Nanjing 210042,China)
关键词:
肠道微生物高通量测序斑点福寿螺
Keywords:
intestinal microbiotahigh-throughput sequencingPomacea maculata
分类号:
Q958
DOI:
10.3969/j.issn.1001-4616.2022.01.010
文献标志码:
A
摘要:
肠道微生物被认为是动物机体的共生体,在营养物质代谢、免疫调控等方面发挥重要作用. 入侵生物斑点福寿螺(Pomacea maculata)严重威胁农业生产和生态系统功能. 本研究利用高通量测序技术对11只雌、雄斑点福寿螺的肠道微生物多样性、结构和功能进行研究. 结果表明,在门水平上,斑点福寿螺肠道内容物主要优势菌为变形菌门(Proteobacteria)(43.52%)和软壁菌门(Tenericutes)(13.82%); 在属水平上,主要优势菌为气单胞菌属(Aeromonas)(15.66%)和支原体属(Mycoplasma)(7.26%). 雌、雄斑点福寿螺肠道微生物多样性和群落结构没有显著性差异(P>0.05). 在斑点福寿螺肠道内容物中检测到一些有益菌以及潜在致病菌. 基于PICRUSt分析预测斑点福寿螺肠道菌群功能,共得到24种代谢功能,其中氨基酸运输和代谢、通用功能预测、信号转导、细胞壁/膜、能量产生和转换丰度较大.
Abstract:
Intestine microbiome are considered as symbionts of animal bodies and play an important role in nutrient metabolism and immune regulation. Pomacea maculata is an invasive species that has caused serious damage to aquatic crop production and ecosystem functioning. In this study,high-throughput sequencing was used to analyze the intestinal microbial diversity,community structure and function of 11 female and male P.maculata. At the phylum level,Proteobacteria(43.52%)and Tenericutes(13.82%)were the dominant bacteria in the intestinal content microbiome of P.maculata. At the genus level,the dominant bacteria were Aeromonas(15.66%)and Mycoplasma(7.26%). No significant difference in intestinal microbial diversity and microbial community structure was found between male and female group(P>0.05). Some beneficial bacteria and potential pathogenic bacteria were found in the intestinal content microbiome of P.maculata. PICRUSt analysis was used to predict the intestinal microbiome functions of P.maculata,and 24 metabolic functions were predicted. The amino acid transport and metabolism,general function prediction only,transcription,cell wall/membrane/envelope biogenesis,energy production and conversion accounted for a large proportion.

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备注/Memo

备注/Memo:
收稿日期:2021-01-14.
基金项目:国家自然科学基金项目(32170434)、江苏省自然科学基金项目(BK20171407).
通讯作者:陈炼,博士,副教授,研究方向:入侵生物学. E-mail:chenlian_2004@163.com
更新日期/Last Update: 1900-01-01