|Table of Contents|

Purification of Acetaldehyde Dehydrogenase From Thermoanaerobacter ethanolicus JW200(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2007年01期
Page:
78-81
Research Field:
生命科学
Publishing date:

Info

Title:
Purification of Acetaldehyde Dehydrogenase From Thermoanaerobacter ethanolicus JW200
Author(s):
Peng Hui1; Mao Zhonggui1; Wu Guogan2; Shao Weilan1; 2*
1.The Key Laboratory of Industrial Biotechnology Under Ministry of Education,Southern Yangtze University,Wuxi 214036,China
2. The Key Laboratory ofM icrob iolE ngineering, Nan jing Norm alUn ivers ity, Nan j ing 210097, Ch in a
Keywords:
ace taldehyde dehydrogenase;  Thermoanaerobacter ethano licus;  pur ification
PACS:
Q814.1
DOI:
-
Abstract:
A aceta ldehyde dehydrogenase ( CoA-acety la ting) w hich is one o f the key enzym es o f the a lco- ho lm etabo lic pathw ay w as pur ified from Thermoanaerobacter ethano licus JW 200 through follow ing steps: ① ion exchang e chrom atography on DEAE-Sepharose Fast Flow, ② amm on ium su lfate fractionation w ith saturation 30% and hydrophob ic in teraction chrom atography on Butyl- H IC, ③ amm onium su lfate frac tionation w ith saturation 80% and hydrophob ic interac tion chrom atography on Bu ty l-H IC, ④ ge l filtration on TOYOPEARL HW - 55F. The pur ified enzyme showed a sing le band on SDS-po lyac ry lam ide ge l e lec tropho resis w ith a pu rifica tion o f 910 fo ld, and a y ie ld o f 7%. The mo lecu la rw e ight o f the subun it and the w ho le enzyme w ere estim ated by SDS-PAGE and g radation PAGE as 100 000 and 360 000, respective ly.

References:

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Last Update: 2013-05-05