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Isolation of Salt-Resistant and Salkali-Resistant Pectate Lyase Producing Strains and Its Cultural Conditions(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2007年04期
Page:
89-93
Research Field:
生命科学
Publishing date:

Info

Title:
Isolation of Salt-Resistant and Salkali-Resistant Pectate Lyase Producing Strains and Its Cultural Conditions
Author(s):
Zhao Qingxin1; 2; Han Fengming2
1.College of Life Science and Biotechnology,Yancheng Teachers University,Yancheng 224002,China
2. K ey  Lab forM icrob ialT echnology in th e S chool of L ife S cien ce, Nan jing Norm alU nivers ity, N an jing 210097, C h ina
Keywords:
sa lina so i; l Bacillus sub tilis;  pectate lyase;  salt-res istant;  salka li-resistant
PACS:
Q93-3
DOI:
-
Abstract:
One pectate lyase producing strain, ZQX8, was separated from the sa lina so il in Yancheng Na tionalGrus japonensis NatureReserve, Jiangsu Prov ince. Through m oda lity of bacteria, spec ia lty in physi o log ic and b iochem ica,l and 16S rDNA, w e identified the bacter ia to be Bacillus subtilis. The results show ed that the m ost optim al cu ltu re conditions of th is bacter ium was: incubating at 37 ℃ , pH 6.5, w ith 0. 1~ 0. 25 m ol/L N aC.l But incubating a t 0.5 ~ 1.0 m o l/L NaC l and pH 7~ 10, the stra in could still g row, and th is showed that the stra in m ight have endurity ag ainst sa lt and sa lka l.i The stra in cou ld secrete pecta te lyase w ith enzym e activ ity 4U /mL med ium. The optium substra te o f th is enzym ew as po lygalactu ron ic ac id and the optium conditions was 40~ 60℃ , pH 8~ 10. The activ ity o f the enzym e still rem a ined at the higher concentration of 600mmo l/L N aC.l The enzym em ight have both sa lt-resistant and sa lka li resistant property.

References:

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Last Update: 2013-05-05