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Comparison and Improvement of Seven Staining Methods Used for the Capsule of Bacillus Mucilaginosus(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2007年04期
Page:
84-88
Research Field:
生命科学
Publishing date:

Info

Title:
Comparison and Improvement of Seven Staining Methods Used for the Capsule of Bacillus Mucilaginosus
Author(s):
Ling Yun; Xiao Zhijie; Lian Bin
1.Jiangsu Key Laboratory of Biological Diversity and Biological Technology,Nanjing 210046,China
2. S chool of L ife S cience, Nan jing N orm alUn ivers ity, N an jing 210046, C h ina
Keywords:
Bacillusm ucilag inosus;  capsule;  sta in ing m ethod
PACS:
Q93-3
DOI:
-
Abstract:
The capsu le is an important kind o f bacterial attachm ent. The ex istence, th ickness and chem i ca l constituents o f the capsu le a re important characters o f bacter ia. Capsu le sta in ing and observ ing are basic skills in bacte rial certification. It needs proper sta ining me thods and pro fic iency to m ake sta in ing slide o f capsule better, because the capsule has low refraction and low affinity w ith dyes. Bacillus muci lag inosus is character ized by its abundant capsu le, and m ost useful pe rform ances o f the bacte ria relate to its capsu les. W e stud ied seven m ethods o f nega tive sta in ing of bac terial capsule and compared their ad vantages and w eaknesses. The resu lts show ed that the ink staining m ethod and Congo red /hydroch lo ric acid sta in ingm ethod are simp le and convenient as w e ll as fast and effective; the im proved Congo red /hy droch lo ric acid sta in ing m ethod m akes the stainingm ore pe rfect, and th is m ethod is w orthy o f popular iza tion in the related lab.

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Last Update: 2013-05-05