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RAPD Analysis of Genome DNA on the Amictic Female and Male of Brachionus calyciflorus(Rotifera:Monogononta)(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2008年03期
Page:
109-112
Research Field:
生命科学
Publishing date:

Info

Title:
RAPD Analysis of Genome DNA on the Amictic Female and Male of Brachionus calyciflorus(Rotifera:Monogononta)
Author(s):
Li Daming; Lu Jianming; Yang Jiaxin; Xu Xiaofeng
School of Life Science,Nanjing Normal University,Nanjing 210097,China
Keywords:
B rachionus calycif lorus;  RAPD;  am ictic fem a le;  ma le;  gene tic background
PACS:
Q953
DOI:
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Abstract:
In o rde r to acqure the know ledge o f the genetic backgrounds wh ich lead to the sex differentiation o fBrachionus caly cif lorus( Ro tifera), the genom ic DNA of am ictic fem ale and m ale o fB rachionus calycif lorus w as analyzed by RAPD techno logy. One o rm ore spec ia l bands w as g enerated by 10 prim ers of 30 tested pr im ers in am ictic fema le o rm a le rotifer and it showed the genetic backg rounds difference between am ictic fema le and m ale. By repeated testing, spec ial bands can be produced stably by pr im ers B15 and B18 respectively in am ictic fem a le and m a le ro tifer. The spec ia l band w as separated, c loned and sequenced. The sequence resu lts w ere subm itted to GenB ank and the accession num bers w ere: AY907696 and AY898611. W e found there were no any high hom ology sequence m atching to the tw o sequences and it showed that they cou ld be new DNA sequence.

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Last Update: 2013-05-05