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Comparison in Tissue Culture Power Between Anther in Indica Rice Yangdao 6 and Japonica Rice Suhuxiangjing(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2008年04期
Page:
108-113
Research Field:
生命科学
Publishing date:

Info

Title:
Comparison in Tissue Culture Power Between Anther in Indica Rice Yangdao 6 and Japonica Rice Suhuxiangjing
Author(s):
Li Xia; Zhao Yuntao; Yan Lina; Zhou Yuelan
Institute of Foods Crop,Jiangsu Academy of Agriculture Sciences,Nanjing 210014,China
Keywords:
indica and jap onica rice;  anther culture;  tissue culture power
PACS:
S511.2
DOI:
-
Abstract:
The cu lture ab ility o f ca llus from anthe r of two rice var ie ties, Ind ica R ice Yangdao 6 and Jap onica R ice Suhux iang jing are stud ied asm ate rials. The results show that when usingM 8 as bas ic induc tion m ed ium adding 60 g /L suc rose, 6 g /L agar, 1m g /L 2, 4- D, 3m g /L NAA, 1m g /L KT, 100m g /L pro line and 300m g /L hydro ly zed case in prote in, inductiv ity o f callus in Y angdao 6 is h igh as 81 5 pe rcents after chilling trea tm ent fo r 9d during 26℃- 31℃ a ir tem pe ra ture fo r samp ling. W hile that in Suhux iang jing is high as 15.05 percents when us ingM 8 as basicm ed ium adding 60 g /L sucro se, 8 g /L aga r, 1 mg /L 2, 4- D, 3 m g /L NAA, 1 mg /L KT, 100 mg /L pro line and 300 m g /L hydro ly zed casein prote in; a fter chilling treatment for 12 d du ring 27℃ - 30℃ a ir temperature for sam pling. And that when using M S as d ifferen tiation basic med ium adding 0.2m g /L 2, 4- D、2m g /L 6- BA、2m g /L KT, the frequency of regeneration o f ca llus from anher in Yangdao 6 and Suhux iang jing are 2.7 percents and 5.7 percents, respec tive ly. The stab le and e-f fective regeneration from anther in Yangdao 6 and Suhux iang jing are established。

References:

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Last Update: 2013-05-05