|Table of Contents|

Expression of H5HA Avian Influenza Virus Gene in Potato Plant(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2009年03期
Page:
104-108
Research Field:
生命科学
Publishing date:

Info

Title:
Expression of H5HA Avian Influenza Virus Gene in Potato Plant
Author(s):
Tan Tian 1; 2 ; Ji Qin 1; Dou Bingde 1; Wang Dengzhan 2; Gu Ke 1
1. Jiangsu Key Laboratory for Eco-Agricultural and Biotechnology Around Hongze Lake,Department of Biology,Huaiyin Normal University,Huaian 223300,China
Keywords:
H 5HA gene;  hem agg lu tinin;  transgen ic potato
PACS:
S532
DOI:
-
Abstract:
In th is study, we exp lo re the possibility o fH 5HA gene expression in po tato plant. TheH5HA-encod ing cDNA fragm en t is fused to the sequence o f CaMV35S prom ote r. One pota to cultiv ar( cv. Desiree) is used forAgrobacter iumm ed iadate transfo rm ation w ith pB IN19 /35S-H 5HA. Twe lve transg en ic, roo ting shoo ts are obta ined and m ultip lied. The expression of H 5HA gene in transgenic plants is analyzed by PCR, RT-PCR andW este rn dot-b lotting. The resu lts show tha tH 5HA gene is successfully expressed in the eleven transfom ed po tato plants, suggesting the possib ility of using po tato as a b ioreactor fo r production o f ora l av ian flu vacc ine.

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Last Update: 2013-04-23