|Table of Contents|

Study on the Active Site of MetMbase Electron Transport out of Cell(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2009年04期
Page:
59-64
Research Field:
化学
Publishing date:

Info

Title:
Study on the Active Site of MetMbase Electron Transport out of Cell
Author(s):
Wang Wei; Jin Bangquan; Lu Yan; Liu Jinlin
Department of Food Science and Nutrition,Nanjing Normal University,Nanjing 210097,China
Keywords:
M eMt base;  e lec tron transport;  NADH;  NaN 3;   ; N-Ethy lm a le im ide;  d iva lent m eta l ions
PACS:
R341
DOI:
-
Abstract:
M eMt base extrac tion from porc ine cardiac m usc le w as studied on its enzym e b io chem ica l properties. Fo r study ingM eMt base active site, NADH, ATP, NaN3, N-E thy lm a le im ide and d iva lentm e tal ions w ere used as b lo ckers o r agon ists in th is research. The resu lts show ed that the express ion o fM eMt base ac tiv ity depended on NADH starting. The m ost optimum NADH concentration w as 0.2mM so that NADH w as a promo tor forM eMt base. In the reaction system, M eMt base activ ity w as inhib ited by the h igher ATP leve ,l because ATP cou ld block e lec tron transport in resp ira tory cha in. A t the third site M eMt base activ ity w as blocked by NaN3. M eMt base was inac tive through N-E thy lm a le im ide bond ing the sulfhydry l g roup( - SH ) in enzym e prote in structure at them om ent. In addition, M eMt base ac tiv ityw as inter fered by som e div alent m eta l ions, such as Zn 2+ , Cu 2+ andM g 2+ . M eMt base activ ity w as increased by Zn 2+ , wh ile it was decreased by Cu 2+ andMg 2+ . The inhib ition ofM g 2+ w as mo re than that of Cu 2+ . The freem e tal ions could be blocked by EDTA as a m eta l chelato r. When EDTA w as added in reaction sy stem, M eMt base activ ity w as expressed enough.

References:

[ 1] Dean R W, Ba ll C O. Ana lys is of them yog lobin fractions on the surface o f bee f cut[ J]. Food Techno logy, 1960, 14: 271-285.
[ 2] A rihara K, Cassens R G, et a .l Localization o f m etm yog lob in-reducing enzym e ( NADH-cytochrom eb5 reductase ) system com ponents in bov ine skeleta lm usc le[ J]. M eat Sc ience, 1995, 39: 205-213.
[ 3] M ikke lsen A, Juncher D, Skibsted L H. M e tm yog lob in reduc tase activ ity in ov ine m. Long issimusdorsi muscle[ J]. M eatScience, 1999, 51: 155-161.
[ 4] 汤祥明, 金邦荃, 曹鹏, 等. 猪心肌中高铁肌红蛋白的提取和纯化[ J] . 畜牧与兽医, 2006, 38( 7): 34-36.
[ 5] Ledw ard D A. Colour o f Raw and CookedM ea t. The Chem istry o fMuscle-based Foods[M ]. Cam bridge: The Roya l Soc iety of Chem istry, 1992: 33-68.
[ 6] Bekh it A D, Geesink G H, I lianM A, et a .l Particu la tem etmyog lob in reduc ing activ ity and its re lationship w ith m eat co lo r[ J]. J Agr icultural and Food Chem istry, 2003, 51( 20): 6 026-6 035.
[ 7] Tang Jia l,i C am eron Faustm a ,l R ichard A, et a.l M ito chondrial reduction of m etm yog lob in: dependence on the electron transpo rt cha in[ J]. Journal of Ag ricu ltura l and Food Chem istry, 2005, 53( 13): 5 449-5 455.
[ 8] 王玮, 汤祥明, 金邦荃. 高铁肌红蛋白、高铁肌红蛋白还原酶与冷鲜肉肉色稳定性的研究[ J]. 食品科学, 2008, 29( 7):94-97.
[ 9] E cheva rne C, RenerreM, Labas R. M etm yog lobin reductase ac tiv ity in bov inem uscles[ J] . M eat Science, 1990, 27: 161-172.
[ 10] Bekhit A E D, Geesink G H, M orton J D, et a.l M etmyog lob in reducing activ ity and co lour stability o f ov ine long issimus muscle[ J]. M ea t Sc ience, 2001, 57( 4) : 427-435.
[ 11] W arr iss P D. M eat Science: An In troducto ry Text[M ]. N ew York: CAB I Pub lishing, 2000.
[ 12] 王镜岩, 朱圣庚, 徐长法. 生物化学[M ] . 北京: 高等教育出版社, 2002: 128-129, 140-141.
[ 13] 俞秀玲, 朱忠勇. 猪肝微粒体NADH-细胞色素b5还原酶的纯化及特性分析[ J] . 生物化学与生物物理学报, 1994, 26( 3): 297-302.
[ 14] W a tts BM, Kendr ick J, ZipserM W, et a.l Enzym atic reducing pathw ays in m eat[ J]. Journa l o f Food Science, 1966, 32:855-862.
[ 15] Pong C Y, Chiou T K, N ieh F P, et a.l Purification and character ization o fm etmyog lob in reductase from o rd inarym usc le of blue- fin tuna[ J]. F isher ies Sc ience, 2000, 66( 3) : 599-604.
[ 16] H ag ler L, Coppes J. M e tm yog lob in reductase identification and pur ification of a reduced n ico tinam ide aden ine d inuc leo tidedependent
enzym e from bov ine hea rtw hich reduces m etm yog lobin[ J] . Journal o f B io log ical Chem istry, 1979, 254 ( 14):6 505-6 514.

Memo

Memo:
-
Last Update: 2013-04-23