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Comparison of Ultrastructure of Callus During Induction of Japonica Rice Plants(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2009年04期
Page:
103-109
Research Field:
生命科学
Publishing date:

Info

Title:
Comparison of Ultrastructure of Callus During Induction of Japonica Rice Plants
Author(s):
Yan Lina1; 2; Li Xia1
1.Institute of Foods Crop,Jiangsu Academy of Agricultural Sciences,Jiangsu High Quality Rice R&D Center,Nanjing 210014,China
Keywords:
J aponica rice;  m ature embryo;  ca llus tissue;  ultrastructure
PACS:
S511.22
DOI:
-
Abstract:
M ature embryos from 3 Jap onica rice va rieties as explan ts w ere inoculated in induction m ed ium (M 8+ 30 g /L Sucrose+ 10 g /L Agar+ 5 g /L So rb ito l+ 2 m g /L 2, 4-D + 0. 3m g /L 6-BA), and the callus inductiv ities of these m ater-i a ls w ere different significantly at 0.05 or 0.01 leve,l respectively. A t the sam e tim e, the ca llus incuba ted at 15、22、29 and 36 d respective ly w ere observered by transm ission e lec tron m icroscop ic. The resu lts showed that these r icem aterials deve loped differently at the sam e induction per iod. The callus ce lls of Yangfujing 7 developed faster to m a ture stage; Wh ile those inW uyujing 3 w ere re lative ly slow er, still keep ing g lucosem etabo lism fo r a long time; H owever the deve-l opment of Suhux iang jing a t the sam e period w as slow est. These pe rfo rm ances in callus ultrastructurew ere also consistent w ith the changes o f d iffe rent induc tiv ities in these varieties. It suggested that the ca llus inductiv ities in Japonica rice have c lose relation w ith ce ll deve lopm ent rate, physio log ica l function and senescence as w el.l

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Last Update: 2013-04-23