|Table of Contents|

Construction of an E.coli-Streptomyces-Pseudomonas Shuttle Expression BAC Vector(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2010年02期
Page:
104-108
Research Field:
生命科学
Publishing date:

Info

Title:
Construction of an E.coli-Streptomyces-Pseudomonas Shuttle Expression BAC Vector
Author(s):
Huang Huiying; Song Jie; Shang Guangdong
Nanjing Engineering and Technology Research Center for Microbiology,Jiangsu Key Lab for Biodiversity and Biotechnology,School of Life Science,Nanjing Normal University,Nanjing 210046,China
Keywords:
BAC vector;  recomb inee ring;  shuttle expression
PACS:
Q78
DOI:
-
Abstract:
H etero logous express ion o f biosynthetic gene c luster is an im po rtant study area o f drug resea rch and development, and it can be used fo r gene function study, to obta in targe t compound o r its de riv atives. A s b iosynthetic gene clus  ter is usua lly very large, no rma l DNA vectors m ay have the prob lem s o f low content, hard to handle because of vector s low copy number or no t su it for shuttle express ion in diffe rent hete ro logous stra ins. In th is wo rk, recom bineer ing strategy as we ll as no rm al gene c lon ing techn iques are used to clone functional hetero logous expression DNA elem ents into pECBAC1, one o f them ost commonly used BAC library construc tion vector.

References:

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Last Update: 2013-04-08