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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:

2010年03期

Page:

66-69

Research Field:

化学

Publishing date:

Info

Title:

Kinetic Study of Urease

Author(s):

Ma Li¹; Qiu Zuming²; Qiu Yexian³

1.Supervision and Evaluation Centre of Teaching,Anhui University of Technology,Ma’anshan 243002,China 2. Jingzhou Cu ltural Relic Preservation C enter, Jingzhou 434020, Ch ina 3. C en tralLaboratory of B io logy Experim ent, Su zhouUn ivers ity of S cien

Keywords:

urease;  enzym e activ ity;  optima-l pH

PACS:

Q936

DOI:

-

Abstract:

The dynam ic property of asperg illus urinary enzyme was investigated w hen urea was used as substra te. The va lue ofKm and Vm ax w ere tested under different pH cond itions( 5. 0, 516, 6.0, 6.6, 7.0, 7.6, 8.0, 8. 6, 9.0). The results showed that the va lue o f pH c lear ly affected theVmax . Vm ax show ed be ll shape in the range of pH 5. 5 to pH 8.0. The enzym ic reaction rate reached m ax imum as pH = 7. 0. The value ofK m was not influenced by pH va lue. The v alues o fKm and Vm ax unde r d ifferent pH ( 5.0, 5.6, 6.0, 6. 6, 7. 0, 7. 6, 8. 0, 8.6, 9.0) showed tha t the affinity to the substrate reached m ax im um when pH = 7. 6 and thus becam e the optim um pH. Th is ur inary enzym e exh ibited good stability in the range o f pH 5. 5 to pH8. 0.

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Last Update: 2013-04-08