|Table of Contents|

Effect of Hyperosmotic Stress on Expression of Soluble SBD2 Recombinant Protien in Escherichia coli(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2010年03期
Page:
70-75
Research Field:
生命科学
Publishing date:

Info

Title:
Effect of Hyperosmotic Stress on Expression of Soluble SBD2 Recombinant Protien in Escherichia coli
Author(s):
Ji Qin1; Zhang Yunfeng1; Luo Yuming1; Xu Chun1; Huang Cuixiang2
1.Jiangsu Key Laboratory for Eco-Agricultural and Biotechnology Around Hongze Lake,School of Life Sciences,Huaiyin Normal University,Huaian 223300,China 2. Jiangsu Key Laboratory for B iodivers ity and B iotechnology, Schoo l ofL ife Scien ce, Nan j ing N
Keywords:
SBD2 pro te in;  compatib le solute;  hyperosmo tic stress;  so luble expression
PACS:
Q786
DOI:
-
Abstract:
An artificial tandm repeat o f a starch-binding dom a in ( SBD2) was eng ineered by tw o copies o f the SBD derived from Bacillus circulans cyc lodex tr in g ly cosy ltransfe rase v ia a linke r peptide. The SBD2 gene was inserted into an E. coli expression v ector, pTrcH is B, to obtain the pTrcH is B /SBD2 vec to r. Recomb inantE. coli Top 10 ce lls, conta-i n ing the pTrcH is B /SBD2construc t, w ere g rown in LB w ith 50μ g /mL am picillin and LB w ith 50μg /mL amp icillin, 1 mo l/L sorb itol and 2.5 mm o l/L g lycy l beta ine, respective ly. Express ion o f SBD2 pro te in w as induced by add ing o f IPTG and ana lyzed by SDS-PAGE. The results show that la rge am ounts of so luble pro tein w ere obta ined by g row ing and induc ing the cells under osmo tic stress in the presence o f so rb ito l and g lycy l beta ine.

References:

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Last Update: 2013-04-08