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Effects of Polysaccharides from Pleurotus Cornucopiae on Ultrastructure Change of Acute Alcoholic Injuried Liver in Mice(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2013年01期
Page:
85-89
Research Field:
生命科学
Publishing date:

Info

Title:
Effects of Polysaccharides from Pleurotus Cornucopiae on Ultrastructure Change of Acute Alcoholic Injuried Liver in Mice
Author(s):
Tao Mingxuan; Zhou Bin; Guo Yongyue; Zhao Yunxia
Jinling College,Nanjing Normal University,Nanjing 210097,China
Keywords:
Pleurotus cornucopiae polysaccharides; acute liver injury; liver tissue; ultrastructure
PACS:
TS201.3
DOI:
-
Abstract:
Objective:The present study investigated the protective effect of polysaccharide from Pleurotus cornucopiae(PCNP)on electron microscopic changes in acute alcoholic hepatic injury in mice.Methods:The 84 mice were randomly divided into blank control group,alcoholic model groups.The mice in alcoholic model groups were administered by gavage with BIFENDATE(150 mg/kg·bw)or PCNP((100、200、400)mg/kg·bw)for 30 d before the administration with dose of 50% alcohol(12 mL/kg·bw).After the last alcohol treament for 12 h,in order to observe the ultrastructural changes of liver cells,all mice were dislocation death to collect samples for preparing ultrathin section for electron microscopy.Result:In model group,hepatic cells exhibited in different degrees of karyopycnosis and the nuclear membranes were irregular; The pathological changes in mitochondria had been clearly observed.In the hepatic cell,vacuoles can be seen and glycogen was increased.The rough endoplasmic reticulum were found as expansion and degranulation; Many large lipid droplets were found around the nucleus and microvilli of bile duct lost.In the PCNP groups,karyolemma were uneven.There were less expansion of mitochondria and less glycogen deposition compared with other groups; In addition,endoplasmic reticulum was not obvious outspread and lipid droplets reduced in cells.Conclusion:It can be seen that PCNP has the protective effect on hepatic ultrastructure induced by alcohol.

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Last Update: 2013-03-31