|Table of Contents|

Association of PPARγ2 Bsr1 Locus Polymorphism in Longissimus Dorsi With Meat Quality Traits in Swine(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2013年03期
Page:
81-86
Research Field:
生命科学
Publishing date:

Info

Title:
Association of PPARγ2 Bsr1 Locus Polymorphism in Longissimus Dorsi With Meat Quality Traits in Swine
Author(s):
Zhu Yun1; Jin Bangquan1; Gong Lan1; Zhang Xixia2; Yuan Yonggang2; Zhou Xin1; Xu Xiaobo3
(1.Department of Food Science and Nutrition,Nanjing Normol University,Nanjing 210023,China) (2.Institute of Animal Husbandry and Poultry Science of Nanjing,Nanjing 210036,China) (3.Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
Keywords:
PPARγ2; Bsr1 locus; polymorphism; meat quality traits; swine
PACS:
S813.1
DOI:
-
Abstract:
Longissimus dorsi(LD)in swine(7 breeds),was used to study PPARγ2 gene and its Bsr1 locus(located in PPARγ2 promoter 1)polymorphism with meat quality traits via RT-PCR,PCR-RFLP and sequencing technology.The result showed that the expression of PPARγ2 was low in swine LD.Its correlation with intramuscular fat was weak(R=0.22).It was also found that the base G in wild type b(CCAGT)was replaced by A.b type of Bsr1 locus would be changed to B type(CCAAT)thus it formed b,Bb and B type.Shanzhu LD had 3 genotypes,while its crossbreds had a low frequency of b type or even no b type.We also noticed that there was a high frequency of Bb type expressed in Shennong 1 and no b type in Landrace and Yorkshire.GLM model was used to analyze the association of genotypes(as a major factor)with meat quality traits.It was indicated that genotypes of PPARγ2 Bsr1 locus were correlated with the shear force of meat and intramuscular fat,significantly(P<0.05).This suggested that the high expression of b type contributed to adipose differentiation,deposition and appropriate for meat tenderness.It also means that PPARγ2 gene can be the meat target gene.

References:

[1] Walczak R,Tontonz P.PPAR adigms and PPAR adoxe:expanding roles for PPAR gamma in the control of lipid metabolism[J].J of Lipid Res,2002,43:177-186.
[2]Maex N,Duez H,Fruchart J C,et al.Peroxisome proliferator activated receptors and atherogenesis:regulators of gene expression in vascular cells[J].Circ Res,2004,94(9):1 168-1 178.
[3]龚兰,金邦荃,章熙霞,等.杂交山猪骨骼肌PPARγ、RARα基因与脂代谢关系的研究[J].食品科学,2011,32(11):62-67.
[4]Matsusue K,Peters J M,Gonzalez F J.PPAR b/d potentiates PPARγ-stimulated adipocyte differentiation[J].FASEB J,2004,18:1 477-1 479.
[5]高勤学,李俊,刘梅,等.猪PPAR基因多态性与脂肪性状相关的初步研究[J].江苏农业科学,2004(2):60-62.
[6]Fan Y Y,Zan L S,Fu C Z,et al.Three novel SNPs in the coding region of PPARγ gene and their associations with meat quality traits in cattle[J].Mol Bio Reports,2011,38:131-137.
[7]Grindflek E,Hoen N,Sundvold H,et al.Investigation of a peroxisome proliferator-activated receptor gamma haplotype effect on meat quality and carcass traits in pigs[J].Anim Genet,2004,35(3):238-241.
[8]Chen Z,Zhao S F,Jiang X L,et al.Association of PPARγ2 polymorphisms with carcass and meat quality traits in a Pietrain×Jinhua F2 population[J].Genet Mol Bio,2011,34(1):56-61.
[9]Schenkel F S,Miller S P,Jiang Z,et al.Association of a single nucleotide polymorphism in the calpastatin gene with carcass and meat quality traits of beef cattle[J].J of Anim Sci,2006,84(2):291-299.
[10]刘兴余,金邦荃,詹巍,等.猪肉嫩度测定方法的改进[J].中国农业科学,2007,40(1):167-172.
[11]Grindflek E,Sundvold H,Lien S,et al.Physical and genetic mapping of the peroxisome proliferator-activated receptor γ gene to porcine chromosome 13[J].J Anim Sci,2002,22(1):1-23
[12]王博,吴江维,杨公社.PPARγ在八眉猪不同组织中的表达差异[J].畜牧兽医学报,2008,39(3):273-277.
[13]Rosen E D,Sarraf P,Troy A E.PPAR gamma is required for the differentiation of adipose tissue in vivo and in vitro[J].Mol Cell,2000,4(4):611-617.
[14]Knouit C,Auwerx J.Peroxisome proliferator-activated receptor-γ calls for activation in moderation:lessons from genetics and pharmacology[J].Endocrine,2004,6(3):899-918.
[15]Omi T,Breni B,Spilar K S,et al.Identification and characterization of novel peroxisome proliferator-activated receptor gamma transcriptional variants in pig and human[J].J Anim Breed Genet,2005,122:45-53.
[16]Grindflek E,Sundvold H,Klungland H,et al.Characterisation of porcine peroxisome proliferator activated receptors γ1 and γ2:Detection of breed and age differences in gene expression[J].BBRC,1998,249(3):713-718.
[17]Grindflek E,Szyda J,Liu Z,et al.Detection of quantitative trait for meat quality in a commercial slaughter pig cross[J].Mamm Genom,2001,12(4):299-304.
[18]Rosenson R S.Effects of peroxisome proliferator-activated receptors on lipoprotein metabolism and glucose control in type 2 diabetes mellitus[J].Amer J of Cardio,2007,199:96B-104B.
[19]Evans R M,Barish G D,Wang Y X.PPARs and the complex journey to obesity[J].Nat Med,2004,10:355-361.
[20]Eleonora M,Vittorio T,Daria C,et al.Interaction between PPARγ2 variants and gender on the modulation of body weight[J].Obesity,2008,16(6):1 467-1 470.
[21]Emnett R S,Grindflek E,Rothschild M F,et al.The effects of porcine peroxisome proliferators-activated receptor gamma(PPARG)in Berkshire,Duroc,Hampshire and Landrace breeds[J].Proceedings of the Conference Plant and Animal Genome,2000,8:396-340.

Memo

Memo:
-
Last Update: 2013-09-30