|Table of Contents|

cDNA Cloning and Sequence Analyses of Six Avian Z-Linked Genes from Peking Gecko(Gekko swinhonis)(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2014年01期
Page:
128-
Research Field:
生命科学
Publishing date:

Info

Title:
cDNA Cloning and Sequence Analyses of Six Avian Z-Linked Genes from Peking Gecko(Gekko swinhonis)
Author(s):
Xia Wenjun; Yuan Yahui; He Xiaolie; Li Peng; Yan Jie; Zhou Kaiya
School of Life Sciences,Nanjing Normal University,Jiangsu Key Laboratory for Biodiversity and Biotechnology,Nanjing 210023,China
Keywords:
Gekko swinhonis; sex determination; Z-linked genes
PACS:
Q95
DOI:
-
Abstract:
We sequenced partial cDNA sequences of the homologues of six avian Z-linked genes from Gekko swinhonis by using the RT-PCR approach.Comparing with those obtained from G.hokouensis,the lengths of all these fragments were identical at both nucleotide and amino acid levels except the 5’ region of CHD1,in which there had a deletion of 3 bp,furthermore,sequence similarity was very high(97%-100%).When comparing with other exsiting sequences from Squamata,Testudines and Crocodylia,varying degrees of similarity was found in sequences of eight fragments from six genes.GHR showed the lowest homology,which was concerdant with the relatively high value of ω(Ka/ks).Cloning and chromosomal location of the sex-chromosome linked genes will be helpful to elucidate the origins and evolutionary processes of different sex chromosomes in geckos or even in higher vertebrates.

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Last Update: 2014-03-30