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Glutamine Synthetase of Bacillus subtilis is Applied to Corynebacterium glutamicum Fermentation System to Produce L-Glutamine(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2015年02期
Page:
78-
Research Field:
生命科学
Publishing date:

Info

Title:
Glutamine Synthetase of Bacillus subtilis is Applied to Corynebacterium glutamicum Fermentation System to Produce L-Glutamine
Author(s):
Bai Jing; Wu Tongsiyu; Wang Qi; Yin Zhimin
School of Life Sciences,Nanjing Normal University,Institute of Biochemistry and Biological Products, Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology,Nanjing 210023,China
Keywords:
Corynebacterium glutamicum; promoter tac; promoter mal; Glutamine synthetase
PACS:
Q789
DOI:
-
Abstract:
Glutamine synthetase(GS)is the key enzyme responsible for L-glutamine(L-Gln)synthesis. However,enzymatic activity of GS is influenced by many factors,leading to the low enzyme activity and the low production of L-Gln. In this study,in order to improve the yield of L-Gln,a high-effective expression system was constructed in Corynebacterium glutamicum with glnA gene. We tested two different strong promoters,promoter tac(Ptac),and promoter mal(Pmal),for a comparison of the activity of GS. After then,for avoiding the adenylylation in C.glutamicum,we constructed plasmids containing the point mutation of glnA,in which Tyr405 is replaced by a phenylalanine residue. Since the glutamine synthetase in Bacillus subtilis is not regulated via adenylylation,we also compared C.glutamicum GS activity with Bacillus subtilis’s to detect the enzyme activity in the given system. Light point of this study is to express GS of Bacillus subtilis in C.glutamicum expression system for the first time. The results reveal that promoter tac was more capable for producing L-Gln in C.glutamicum system. GS enzyme from Bacillus subtilis had the higher activity than C.glutamicum’s. The recombinant strain BJ2 has the highest GS activity and produce 32.5 g/L L-glutamine.

References:

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Last Update: 2015-06-30