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The Impact of Carbon，Nitrogen Source as well as pH Value on the Growth and Biotransformation Activity of Lysinibacillus fusiformisCGMCC 4913，an Organic Solvent-Tolerant Glycosylationof Puerarin Producing Strain Organic(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2016年02期
Page:
61-
Research Field:
生命科学
Publishing date:

Info

Title:
The Impact of Carbon，Nitrogen Source as well as pH Value on the Growth and Biotransformation Activity of Lysinibacillus fusiformisCGMCC 4913，an Organic Solvent-Tolerant Glycosylationof Puerarin Producing Strain Organic
Author(s):
Liu Guiyou1; 2; Wang Siyuan2; Yuan Sheng2
（1.Faculty of Life Science and Chemical Engineering，Jiangsu Second Normal University，Nanjing 210013，China）（2.School of Life Sciences，Nanjing Normal University，Jiangsu Engineering and Technology Research Center for Industrialization ofMicrobial Resources
Keywords:
puerarin; organic solvent-tolerant; Lysinibacillus fusiformis; culture conditions
PACS:
Q939.99
DOI:
10.3969/j.issn.1001-4616.2016.02.012
Abstract:
An organic solvent-tolerant Lysinibacillus fusiformis CGMCC 4913，isolated from the local soil sample，has the ability to convert puerarin into puerarin-7-O-fructoside by the biotransformation. This study aimed to clarify the effects of carbon sources，nitrogen sources and pH on the growth and transforming activity of L. fusiformis CGMCC 4913. The results indicated that LB medium can totally act as carbon source for L. fusiformis CGMCC 4913. And the transformation activity of the strain taking peptone and tryptone as nitrogen source performs good. LB cultured Lysinicillus fusiformis CGMCC 4913 strain grows well and exhibits relatively high and stable transformation activity.

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Last Update: 2016-06-30