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The Effect of Antioxidant Activity on the Polysaccharides fromPleurotus cornucopiae with Different Extraction Ways(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2016年04期
Page:
0-
Research Field:
·生命科学·
Publishing date:

Info

Title:
The Effect of Antioxidant Activity on the Polysaccharides fromPleurotus cornucopiae with Different Extraction Ways
Author(s):
Wang Meiju; Lu Wenjuan; Yu Chen; Tao Mingxuan
College of Ginling,Nanjing Normal University,Nanjing 210097,China
Keywords:
polysaccharides from Pleurotus cornucopiae; extraction yield; antioxidant activity; free radicals
PACS:
TS201.4
DOI:
10.3969/j.issn.1001-4616.2016.04.012
Abstract:
Seven different extaction methods of the polysaccharides from pleurotus cornucopiae,including traditional hot water extraction,ultrasonic extraction,ultrasonic wave complex hot water extraction,acid extraction,alkali extraction,ultrasonic wave complex acid extraction and ultrasonic wave complex alkali extraction. And antioxidant activity of the polysaccharides in virto was investigated by using reducing power,hydroxyl radical scavenge activity,superoxide radical assay and DPPH· scavenge activity,using Vc as positive control. The results showed that the polysaccharides prepared by acid extraction had the highest extraction yield that reached 6.58%. In general,the polysaccharides by ultrasonic wave complex hot water extraction reached the highest reducing power(2.18 at 10 mg·mL-1),hydroxyl radical scavenging ability was(95.56% at 10 mg·mL-1),Superoxide anion radical scavenging activity was(62.35% at 1 mg·mL-1),DPPH· scavenging ability was(80.32% at 2 mg·mL-1). The antioxidant activity of the polysaccharides prepared by ultrasonic wave complex alkali extraction was also strong,but the polysaccharides prepared by ultrasonic wave complex acid extraction was the worst. It is concluded that ultrasonic-assisted hot water extraction is an effective method for extracting polysaccharides from pleurotus cornucopiae,and it’s more conducive to study the biological activity of polysaccharides.

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Last Update: 2016-12-31