|Table of Contents|

Primary Culture and Identification of Mouse BrainMicrovascular Endothelial Cell(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2017年03期
Page:
128-
Research Field:
·生命科学·
Publishing date:

Info

Title:
Primary Culture and Identification of Mouse BrainMicrovascular Endothelial Cell
Author(s):
Song Xiaobin1; Fan Chun’e1; Wang Ye2; Zhang Yiteng1; Cui Congcong1; Zeng Weiwei1; Zheng Lirong1; Cai Xiaojie1; Chen Huaqun1
(1.School of Life Sciences,Nanjing Normal University,Jiangsu Key Laboratory for Molecular and Medical Biotechnology,Nanjing 2l0023,China)(2.Nanjing Mucyte Biotechnical Com.,Nanjing 210061,China)
Keywords:
mouse brain microvescular endothelial cell; primary culture; density gradient centrifugation; enzyme digestion
PACS:
Q28
DOI:
10.3969/j.issn.1001-4616.2017.03.021
Abstract:
Brain microvascular endothelial cell(BMVEC)is an essential component of the brain blood barrier(BBB). It has been known that the dysfunction of BBB plays critical roles in the pathological process of a variety of neurological disorders. The primary cultured BMVECs were widely used in the study of the functions and the regulation mechanisms of the BBB. Here,it is reported that a simple and effective method of isolating and growing of BMVEC established upon density gradient centrifugation and enzyme digestion. The purity of the cultured cells was higher than 95% determined by CD31 immunofluorescence staining. The features of the cells were further confirmed by positive staining of VE-cadherin and ZO-1,the characteristics of BMVECs. Our method may provide a good approach for the study of BBB in vitro.

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Last Update: 2017-09-30