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¡¶ÄϾ©Ê¦´óѧ±¨£¨×ÔÈ»¿Æѧ°æ£©¡·[ISSN:1001-4616/CN:32-1239/N]

Issue:

2018Äê01ÆÚ

Page:

61-

Research Field:

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Publishing date:

Info

Title:

Interaction of Luciferase and Hyp-Mg²⁺

Author(s):

Ding Yi; Li Xiaoting; Zhou Lin; Zhou Jiahong

School of Chemistry and Materials Science,Nanjing Normal University,Nanjing 210023,China

Keywords:

Hypericin; Luciferase; UV-Vis; fluorescence spectroscopy; CD spectroscopy

PACS:

O657.39

DOI:

10.3969/j.issn.1001-4616.2018.01.012

Abstract:

The interaction between luciferase(Luc)and Hyp-Mg²⁺ complex was studied by UV-Vis absorption spectroscopy,fluorescence spectroscopy and circular dichrosim(CD)spectroscopy. The UV-Vis studies present that Hyp-Mg²⁺ can interact with Luc and disturb the structure of Luc. Fluorescence spectroscopy revealed that the binding constant of Luc with Hyp-Mg²⁺ complex at 298 K is 3.13¡Á10⁵ L/mol and Hyp-Mg²⁺ can quench the fluorescence of Luc through the static quenching mechanism. CD spectra indicate Hyp-Mg²⁺ can change the molecular conformation of Luc.

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