|Table of Contents|

FgPDK1 Modulates Osmotic Stress Responses in Fusarium graminearum(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2018年01期
Page:
76-
Research Field:
·生命科学·
Publishing date:

Info

Title:
FgPDK1 Modulates Osmotic Stress Responses in Fusarium graminearum
Author(s):
Li Haimei1; Wang Yingying1; Gao Tao2; Xu Cunfa3; Shi Zhiqi2; Chen Jian2; Xu Xiaofeng1
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)(2.Institute of Food Safety and Nutrition,Jiangsu Academy of Agriculture Science,Nanjing 210014,China)(3. Central Laboratory,Jiangsu Academy of Agriculture Science,Nanjing 210014,
Keywords:
Fusarium graminearum; PDK; osmotic stress; glycerol; cell death
PACS:
S432.1
DOI:
10.3969/j.issn.1001-4616.2018.01.014
Abstract:
Fungi’s HOG(high osmolarity glycerol)pathway in response to osmotic stress has been revealed. However,the upstream signaling regulator of the pathway remains unclear. In this study,we investigated the pivotal role of a mitochondrial energy metabolic gene encoding for pyruvate dehydrogenase kinase(FgPDK1)in Fusarium graminearum in the regulation of NaCl stress by using genetic and physiological-biochemical approaches. The main results have been obtained as follows,(1)Determination of hyphal growth indicated that ΔFgPDK1(an FgPDK1 knock-out mutant)was more sensitive than wild type(PH-1).(2)Compared to PH-1,NaCl stress induced more electrolyte leakage and higher accumulation of glycerol in the hyphae of ΔFgPDK1.(3)ROS accumulation,MDA content,and cell death increased significantly in ΔFgPDK1 as compared to PH-1 under NaCl stress.(4)The relative expression levels of anti-oxidative genes(SOD and CAT)were significantly lower in ΔFgPDK1 as compared to PH-1 under NaCl stress.(5)All of the above physiological and biochemical responses could be recovered to the level of PH-1 in ΔFgPDK1-C(a gain-of-function complementary strain of ΔFgPDK1). These results suggested that FgPDK1 played important roles in the regulation of HOG downstream pathway and the maintenance of anti-oxidative capacity in F.graminearum under high osmatic stress. This study provides new evidence for understanding the molecular mechanism in fungi’s osmatic stress adaption.

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Last Update: 2018-03-31