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Enzymatic Synthesis of γ-Glutamyl Cysteine with GGT(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2018年02期
Page:
78-
Research Field:
·生命科学·
Publishing date:

Info

Title:
Enzymatic Synthesis of γ-Glutamyl Cysteine with GGT
Author(s):
Wei Guangxu; Tang Yi; Yin Zhimin
School of Life Sciences,Nanjing Normal University,Institute of Biochemistry and Biological Products,Jiangsu Key Laboratory for Molecular and Medical Biotechnology,Nanjing 210023,China
Keywords:
γ-glutamyl transpeptidase; γ-glutamylcysteine; cystine; enzymatic synthesis
PACS:
Q555.3
DOI:
10.3969/j.issn.1001-4616.2018.02.014
Abstract:
Gamma-glutamyl transpeptidase(GGT),which was cloned from the genome of E.coli K-12,was used as a catalyst to synthesis γ-glutamylcystine,with L-glutamine and cystine as the substrate. After the reduction reaction and γ-glutamylcysteine was synthesized. We studied the effects of reaction time,initial enzyme concentration,feed mode of L-glutamine on the enzymatic process and find that the maximal production of GGC would be 6.13 g/L and the conversion rate of L-glutamine would be 24.5%,when the initial reaction conditions of 0.1 mol/L of L-glutamine,0.2 mol/L of cystine and 0.024 U/mL of GGT,and the reaction is carried out at pH 10.0 and 40 ℃ for 12 h.

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Last Update: 2018-11-06