«Previous Article|Table of Contents|Next Article»

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:

2020年01期

Page:

100-106

Research Field:

·生物学·

Publishing date:

Info

Title:

PlasmaMembraneDepolarizationPromotesPrimaryMyoblastProliferationThroughActivationofMAPKSignalingPathway

Author(s):

ZhengLirong; XuYanhua; ZengWeiwei; ChenYuchen; FanChun’e; ChenHuaqun

SchoolofLifeSciences,NanjingNormalUniversity,JiangsuKeyLaboratoryforMolecularandMedicalBiotechnology,Nanjing2l0023,China

Keywords:

depolarization; primarymyoblast; proliferation; MAPKsignalingpathway

PACS:

Q291

DOI:

10.3969/j.issn.1001-4616.2020.01.015

Abstract:

Plasmamembrane(PM)depolarizationcanenhancetheproliferationofcertaintypesofcellsincludingsatellitecell.However,theeffectsofPMdepolarizationontheproliferationofmyoblasthavenotbeendetermined.Inthisstudy,itwasinvestigatedtheeffectsofPMdepolarizationviahighconcentrationofextracellularpotassiumion(K⁺)ontheproliferationofmurineprimarymyoblasts.Bydirectcellcounting,itwasshownanapparentincreaseinthenumberofcellsculturedinmediumcontaining25mmol/LK⁺comparedwiththatofregularmedium(5mmol/LK⁺).However,excessivelyhigher(50mmol/Land100mmol/L)concentrationsofK⁺didnotpromotethegrowthofmyoblasts.Inaddition,adecreaseinthenumberofcellsundergoingG1phaseandanincreaseinthatofSphasewasdetectedbyFACStechnology.ImmunostainingandWesternblotanalysisshowedthatthemyogenicdifferentiationpotentialwasnotalteredafterPMdepolarizationtreatment.Furthermore,itwasfoundthatthebasallevelofactivatedextracellularsignal-regulatedkinase1/2(ERK1/2)wasenhancedwithPMdepolarizationtreatment.BothU0126andPD98059,theMEK1/ERK1/2inhibitors,obviouslyattenuatedtheproliferationenhancementofthecells.Takentogether,ourdataindicatethatPMdepolarizationpromotestheproliferationofprimarymyoblaststhroughactivationofMAPK/ERK1/2pathway.

References:

[1]BLACKISTONDJ,MCLAUGHLINKA,LEVINM.Bioelectriccontrolsofcellproliferation:ionchannels,membranevoltageandthecellcycle[J].Cellcycle,2009,8(21):3527-3536.
[2]SUNDELACRUZS,LEVINM,KAPLANDL.Roleofmembranepotentialintheregulationofcellproliferationanddifferentiation[J].Stemcellreviewsandreports,2009,5(3):231-246.
[3]SUNDELACRUZS,LEVINM,KAPLANDL.Depolarizationaltersphenotype,maintainsplasticityofpredifferentiatedmesenchymalstemcells[J].TissueengineeringpartA,2013,19(17-18):1889-1908.
[4]BALITSKYKP,SHUBAEP.Restingpotentialofmalignanttumourcells[J].Acta-Uniointernationaliscontracancrum,1964,20(3):1391-1393.
[5]CONECD,CONECM.Inductionofmitosisinmatureneuronsincentralnervoussystembysustaineddepolarization[J].Science,1976,192(4235):155-158.
[6]RAYAA,KAWAKAMIY,RODRIGUEZEC,etal.Notchactivityactsasasensorforextracellularcalciumduringvertebrateleft-rightdetermination[J].Nature,2004,427(6970):121-128.
[7]TSENGAS,BEANEWS,LEMIREJM,etal.Inductionofvertebrateregenerationbyatransientsodiumcurrent[J].Journalofneuroscience,2010,30(39):13192-13200.
[8]BUCKINGHAMM.Skeletalmuscleformationinvertebrates[Review][J].Currentopinioningenetics&development,2001,11(4):440-448.
[9]ZHOUY,WONGCO,CHOKJ,etal.Signaltransduction.MembranepotentialmodulatesplasmamembranephospholipiddynamicsandK-Rassignaling[J].Science,2015,349(6250):873-876.
[10]KAHANEN,CINNAMONY,KALCHEIMC.Theoriginandfateofpioneermyotomalcellsintheavianembryo[J].Mechanismsofdevelopment,1998,74(1-2):59-73.
[11]PARTRIDGETA,MORGANJE,COULTONGR,etal.Conversionofmdxmyofibresfromdystrophin-negativeto-positivebyinjectionofnormalmyoblasts[J].Nature,1989,337(6203):176-179.
[12]HUARDJ,BOUCHARDJP,ROYR,etal.Humanmyoblasttransplantation:preliminaryresultsof4cases[J].Muscle&nerve,1992,15(5):550-560.
[13]WEIYIL,YEFEIW,PENGPENGB,etal.Hypoxiapromotessatellitecellself-renewalandenhancestheefficiencyofmyoblasttransplantation[J].Development,2012,139(16):2857-2865.
[14]YUXW,DUMONTNA,RUDNICKIMA.Musclestemcellsataglance[J].Journalofcellscience,2014,127(21):4543-4548.
[15]RANDOTA,BLAUHM.Primarymousemyoblastpurification,characterization,andtransplantationforcell-mediatedgenetherapy[J].Thejournalofcellbiology,1994,125(6):1275-1287.
[16]FENNELLYC,WANGZ,CRISWELLT,etal.Sustaineddepolarizationoftherestingmembranepotentialregulatesmuscleprogenitorcellgrowthandmaintainsstemcellpropertiesinvitro[J].Stemcellreviews&reports,2016,12(6):1-11.
[17]ALLBROOKD.Skeletalmuscleregeneration[J].Muscle&nerve,1981,4(3):234-235.
[18]YOHANNESM,YOHANNEST.HowERK1/2activationcontrolscellproliferationandcelldeath:issubcellularlocalizationtheanswer[J].Bioorganic&medicinalchemistryletters,2009,8(8):1168-1175.
[19]JRCC.Electroosmoticinteractionsaccompanyingmitosisinitationinsarcomacellsinvitro[J].TransactionsoftheNewYorkacademyofsciences,1969,31(4):404-427.
[20]SACHSHG,STAMBROOKPJ,EBERTJD.Changesinmembranepotentialduringthecellcycle[J].Experimentalcellresearch,1974,83(2):362-366.
[21]MAGNIM,MELDOLESIJ,PANDIELLAA.Ioniceventsinducedbyepidermalgrowthfactor.evidencethathyperpolarizationandstimulatedcationinfluxplayaroleinthestimulationofcellgrowth[J].Journalofbiologicalchemistry,1991,266(10):6329-6335.
[22]BOONSTRAJ,MUMMERYCL,TERTOOLENLGJ,etal.Cationtransportandgrowthregulationinneuroblastomacells.ModulationofK⁺-transportandelectricalmembranepropertiesduringthecellcycle[J].Journalofcellularphysiology,2010,107(1):75-83.
[23]FABIOAI,ELISABETTAP,VINCENZOB,etal.Expression,localization,andpharmacologicalroleofKv7potassiumchannelsinskeletalmuscleproliferation,differentiation,andsurvivalaftermyotoxicinsults[J].Journalofpharmacology&experimentaltherapeutics,2010,332(3):811-820.
[24]MOROKUMAJ,BLACKISTOND,ADAMSDS,etal.Modulationofpotassiumchannelfunctionconfersahyperproliferativeinvasivephenotypeonembryonicstemcells[J].ProceedingsofthenationalacademyofsciencesoftheUnitedStatesofAmerica,2008,105(43):16608-16613.
[25]LIZH,ALVAREZVE,GAUDENZIJGD,etal.Hyperosmoticstressinducesaquaporin-dependentcellshrinkage,polyphosphatesynthesis,aminoacidaccumulation,andglobalgeneexpressionchangesintrypanosomacruzi[J].Journalofbiologicalchemistry,2011,286(51):43959-43971.

Memo

Memo:

-

Common functions

Last Update: 2020-03-15