|Table of Contents|

Distribution Characteristics of Microsatellites in theWhole Genome of Cyprinus carpio,Linnaeus(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2021年03期
Page:
103-111
Research Field:
·生物学·
Publishing date:

Info

Title:
Distribution Characteristics of Microsatellites in theWhole Genome of Cyprinus carpio,Linnaeus
Author(s):
Liang Xia; Wang Huiqi; Ma Yuxuan; Song Lei; Wu Chao; Li Lianghui; Zhang Guosong
Physiological,Biochemical and Applied Laboratory,Heze University,Heze 274000,China
Keywords:
Cyprinus carpio; genome; microsatellite; characteristics
PACS:
Q959.46+8
DOI:
10.3969/j.issn.1001-4616.2021.03.016
Abstract:
In this study,the microsatellite search software MISA was used to search for the complete microsatellite in the whole genome of C.carpio,and its distribution pattern was analyzed. Meanwhile,GO annotation enrichment and KEGG enrichment analysis were conducted for the genes located in the coding region of the microsatellite. The results were as follows,837,004 complete microsatellites were searched in the whole genome of C.carpio with a relative abundance of 488/Mb and a total length of 15 513 551 bp,accounting for 0.91% of the whole genome size of C.carpio,with a relative density of 9 051 bp/Mb. Among the 6 complete microsatellites,the number of Mononucleotide was the largest(59.19%),followed by the Dinucleotide(26.79%),Trinucleotide(8.48%),Tetranucleotide(4.21%),Pentanucleotide(1.25%)and Hexanucleotide(0.07%). Among them,A,AC,AT,AAT,AG,C,AAAT,AAC,AGAT,AAG were the top ten microsatellite categories that appeared most frequently in the whole genome of C.carpio,showing obvious A/T base advantage. Through genome-wide microsatellite localization,a total of 24 222 microsatellites were located on gene exons and distributed on 3 853 genes. Among the 1 758 GO entries annotated by GO function,the number of entries and genes annotated by biological process were the most,and the most significant entry enriched by GO function was the regulation of metabolic process. KEGG enrichment showed that the total number of genes enriched in the branches of the organism system was the largest,the accumulation of sphinolipid signaling pathway genes was the most significant in the branches of environmental information processing,and the types of pathways distributed in the metabolic branches were the most extensive. The results suggest that microsatellites located in the gene coding region may play a role in intercellular information communication,signal transduction,cell metabolism and biosynthesis regulation. This study provides data support for the subsequent population genetic information assessment,development of microsatellite primers and functional identification of C.carpio.

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Last Update: 2021-09-15