|Table of Contents|

The Identification,Adaptive Evolutionary Analyses and mRNA Expression Pattern of eIF4G-Like Gene in Larval Development Stages of Eriocheir sinensis(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2022年03期
Page:
96-107
Research Field:
生态学
Publishing date:

Info

Title:
The Identification,Adaptive Evolutionary Analyses and mRNA Expression Pattern of eIF4G-Like Gene in Larval Development Stages of Eriocheir sinensis
Author(s):
Wu Haixia1; Shi Xinye1; 2; Zhou Kaiya1; Yan Jie1; Li Peng1
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)(2.School of Ocean Science and Engineering,Nanjing Normal University,Nanjing 210023,China)
Keywords:
Eriocheir sinensis; eukaryotic translation initiation factors; cDNA cloning; mRNA expression; adaptive evolution
PACS:
Q951+.3
DOI:
10.3969/j.issn.1001-4616.2022.03.013
Abstract:
Eukaryotic translation initiation factor 4 gamma(eIF4G)plays a critical role in the initiation of cap-dependent translation by acting as a scaffolding protein for the assembly of eIF4F complex. To investigate the role of eIF4G gene from the Chinese mitten crab during larval development stages,the full-length cDNA of eIF4G-like gene(denoted as EseIF4G)was cloned and identified by RACE(rapid amplification of cDNA ends)PCR approch,and the expression patterns were detected in larval developmental stages. Furthermore,the present study explored whether adaptive evolution of eIF4G gene occurred during arthropods evolution by site model,branch model and branch-site model implemented in CODEML program of the PAML package,and SLAC(fixes effects likelihood),FEL(fixes effects likelihood)and FUBAR(fast unconstrainted bayesian approximation)methods implemented in Datamonkey website. Bioinformatics analyses revealed that the full-length cDNA of EseIF4G is composed of 3 769 nucleotides with an open-reading frame of 2 379 base pairs,which encoding 792 amino acids and containing MIF4G,MA3 and W2/eIF5C domain. EseIF4G is an unstable,no-secretory and omini-α protein,and has no transmembrane,hydrophobic and signal region. Real-time PCR analysis indicated that the EseIF4G gene was widely expressed in the embryo(O)and nine different developmental stages of larvae(Z1:the first zoeal stage,Z2:the second zoeal stage,Z3:the third zoeal stage,Z4:the fourth zoeal stage,Z5:the fifth zoeal stage,M:megalopa stage,J1:the first juvenile crab stage,J2:the second juvenile crab stage,J3:the third juvenile crab stage). The expression levels of EseIF4G mRNA were relatively higher from the embryo stage to stage Z4,and were relatively lower at stage Z5,M,J1 and J2. The expression levels of EseIF4G mRNA sharply declined to the lowest at stage Z5,which had no significant difference with stage J2(P>0.05),and then increased significantly at stage M and J1(P<0.05). Segregating sites analysis indicated that site 97 and site 457 were the segregating sites of EseIF4G protein,which were located in MIF4G domain and MA3 domain,respectively. Selective pressure analysis showed that overall,there was evidence for strong purifying selection under one-ratio model,with a ω value of 0.0355(significantly less than 1). No positively selected sites were found by site model in PAML,but two shared amino acid sites(448G and 655N)were found by FEL and FUBAR method in Datamonkey. When the terminal branch of E. sinensis was set as foreground,we did not detected any positively selected sites in PAML. Present data suggested that EseIF4G gene involving in the early developmental process of E. sinensis,was possible to serve as a regulator of mRNA translation process and proteins interaction,and was relatively conserved in the evolution of arthropod. These results provide new insights into the mechanisms of brachyurization metamorphosis in decapod crustaceans.

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Last Update: 2022-09-15