|Table of Contents|

Extraction,Carboxymethylation Modification and in Vivo Immunomodulatory Effects of Polysaccharides from Rhizopogon Rubescens(Tul.)Tul(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2023年03期
Page:
60-68
Research Field:
生物学
Publishing date:

Info

Title:
Extraction,Carboxymethylation Modification and in Vivo Immunomodulatory Effects of Polysaccharides from Rhizopogon Rubescens(Tul.)Tul
Author(s):
Huang Xinyue; Ding Huimin; Ma Shixuan; Wang Quiyan; Tao Mingxuan
(School of Food Science and Pharmaceutical Engineering,Nanjing Normal University,Nanjing 210023,China)
Keywords:
Rhizopogon rubescens(Tul.)Tul;  polysaccharide;  carboxymethylation modification;  immunomodulatory activity
PACS:
TS201.2
DOI:
10.3969/j.issn.1001-4616.2023.03.009
Abstract:
Rhizopogon rubescens(Tul.)Tul is a kind of edible fungi of the Rhizopogon,which is mainly distributed in Yunnan and Fujian provinces of China. The primary polysaccharide fraction from Rhizopogon rubescens(Tul.)Tul(PPRR)was extracted by ultrasonic-assisted hot water extraction. Then the PPRR was modified by carboxymethylation to obtain carboxymethyl polysaccharide(CM-PPRR),and the immune activities of the two polysaccharides were compared by animal experiments in vivo. The immune activities of the two polysaccharides were evaluated by the content of serum cytokines,the number of blood cells,the phagocytic ability of mononuclear macrophages and the morphology of organs. The results show that the total sugar content of polysaccharide was 92.06%,and the degree of substitution of carboxymethyl was 0.352. In vivo experiments show that both the PPRR and CM-PPRR can significantly alleviate the decline of organ index,the content of some serum cytokines,the weakening of hematopoietic function and macrophage phagocytosis,and the tissue damage of spleen and thymus caused by cyclophosphamide(CPA),especially CM-PPRR. It showed that both PPRR and CM-PPRR had certain immunomodulatory effects,and CM-PPRR had more significant immune activity in vivo.

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Last Update: 2023-09-15