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Identification of Crystalline Cellulose-Degrading Thermophilic Bacterium and the Characterization of Avicelase(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2007年02期
Page:
84-88
Research Field:
生命科学
Publishing date:

Info

Title:
Identification of Crystalline Cellulose-Degrading Thermophilic Bacterium and the Characterization of Avicelase
Author(s):
Wu Yaohua; Shao Weilan
The Key Laboratory of Microbiology Engineering,Nanjing Normal University,Nanjing 210097,China
Keywords:
M eiotherm us;  16S rDNA;  Avicelase;  enzyme activity;  purification and characterization
PACS:
Q93-3
DOI:
-
Abstract:
A rosy-p igmented Gram-negative, thermophilic bacterium which could p roduce avicelase effi ciently,was isolated from Tengchong hot sp rings in Yunnan p rovince. The strain named TC - 1, was iden tified as the genus ofM eiotherm us, based on the results ofmorphological, physio-biochemical characteris tics, and the homological analysis of 16S rDNA sequence. The TC - 1 strain could utilize crystalline cel lulose, carboxymethylcellulose, xylan and glucose. Experimental investigations revealed that crystalline cellulose could induce p lenty of enzyme p roduction. The enzyme was partially purified, itsmaximal activi ty with the substrate of crystalline cellulose was at pH610 and 70℃, respectively. The studies on pH and temperature stability showed that it was stable enough between pH5.5～6.5 at 70℃ for 1 h, and more than 80% of the activity still remained when incubation was p rolonged to 1 h at 70℃.

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Last Update: 2013-05-05