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Authentication of Crocus sativus L.and its Adulterants by rDNA ITS Sequences and Allele-Specific PCR(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2007年02期
Page:
89-92
Research Field:
生命科学
Publishing date:

Info

Title:
Authentication of Crocus sativus L.and its Adulterants by rDNA ITS Sequences and Allele-Specific PCR
Author(s):
Mao Shanguo1.2; Luo Yuming2; Shen Jie1; Ding Xiaoyu1
1.School of Life Science,Nanjing Normal University,Nanjing 210097,China
2. Department of Biology, Huaiyin Teachers College, Huaian 223300, China
Keywords:
Crocus sativus L. ;  rDNA ITS region;  adulterant;  allele-specific PCR;  molecular authentica tion
PACS:
S567.239
DOI:
-
Abstract:
The PCR p roducts of rDNA ITS regions of Crocus sativus L. and its adulterantswere sequenced and analyzed using Clustal X、Mega 3.0 software. The results showed that the full length of rDNA ITS re gions of C. sativus was 650 bp, and the percentage of GC contents was 60.3% , which disp layed the re markable distinction between rDNA ITS regions of C. sativus and its adulterants. In addition, a pair of al lele-specific PCR (AS - PCR) p rimerswere designed to authenticate C. sativus and its adulterants accu rately without sequencing.

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Last Update: 2013-05-05