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Induced Expression of Recombinant Bacillus Subtilis Glutamine Synthetase in Optimized M9 Medium(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2007年04期
Page:
74-79
Research Field:
生命科学
Publishing date:

Info

Title:
Induced Expression of Recombinant Bacillus Subtilis Glutamine Synthetase in Optimized M9 Medium
Author(s):
Liu Shunyi; Yin Zhimin
School of Life Science,Nanjing Normal University,Nanjing 210046,China
Keywords:
Glutamine Synthetase;  op timizedM9 medium;  p rokaryotic exp ression
PACS:
Q789
DOI:
-
Abstract:
The gene of Bacillus Subtilis Glutamine Synthetase ( GS EC 6.3.1.2 ) was amp lified and cloned into pET3C, then the recombinant p lasmid was transformed into BL21 (DE3). Many types ofM9 medium and LB medium were used respectively to culture the bacteria. The factors such asmedium selec tion, carbon source composition, dose of carbon source, best combination of carbon source and inducer were analyzed in detail. The exp ression efficiency of op timizedM9 medium and LB medium are almost the same, which p rovides evidence that op timizedM9 medium is a p romising rep lacement of LB medium in the industrial p roduction of recombinant Glutamine Synthetase in the future.

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Last Update: 2013-05-05