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A General Method for Direct DNA Vector Modification(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2007年04期
Page:
80-83
Research Field:
生命科学
Publishing date:

Info

Title:
A General Method for Direct DNA Vector Modification
Author(s):
Xie Feng; Zhu Lei; Shang Guangdong
Jiangsu Key Laboratory for Biodiversity and Biotechnology,Nanjing 210046,China
Keywords:
recombineering;  three p ieces cloning;  conjugal transfer vector
PACS:
Q78
DOI:
-
Abstract:
Cloning one ormore DNA fragments into a vector is the one ofmost commonly used techniques in molecular biology. Classic cloning strategy, when dealt with comp licated cloning step s, like multip le fragments insertion, selective marker exchange, are often troubled by the choice of restriction enzymes, the base mutations caused by long fragment PCR, UV exposure and gel purification. We made use of the Red /ET technology to clone two fragments into the vector while remove the unnecessary vector part in a single step. This three p ieces cloningmanipulation is straightforward and convenient, with the potential to be a general cloning strategy for vector engineering.

References:

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Memo

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Last Update: 2013-05-05