|Table of Contents|

Spectroscopic Studies on Interaction of Elsinochrome A With Hemoglobin(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2010年02期
Page:
46-49
Research Field:
化学
Publishing date:

Info

Title:
Spectroscopic Studies on Interaction of Elsinochrome A With Hemoglobin
Author(s):
Ma Fei1; Zhou Lin1; Wang Wei1; Feng Yuying1; Zhou Jiahong1; Wang Xinhe2; Shen Jian1
1.Key Lab of Biofunction Materials of Jiangsu Province,Analysis and Testing Center,Nanjing Normal University,Nanjing 210046,China 2. School of Physics and Technology, Nan jing N orm alUn ivers ity, N an jing 210046, China
Keywords:
Els inochrom e A;  hem og lobin;  UV-v is spectroscopy;  fluorescence spec tro scopy;  synchronous fluorescence spectroscopy
PACS:
R96
DOI:
-
Abstract:
The inte raction between E lsinochrome A ( EA) and hem og lob in ( H b) w as investig ated us ing UV-v is spec troscopy and fluorescence spectroscopy. The resu lts suggested tha t the inte raction m odesw ere obv iously different in the dark and the illum inated cond ition. In the dark, EA inte racted w ith the surface am ino ac id ofH b on ly, but w ith the illum ina tion, bo th am idic ac id and the inte rio r hem achrom e o fHb inte racted w ith EA. A cco rding to the va lues of the quenching constant and the thermodynam ics param eters, the quench ingm echan ism was dynam ic quenching procedure and the inter ac tion between EA and H b w as m a inly hydrophobic in na ture. The chang es in the m icroc ircum stance o f am ino acid o f Hb w ere analyzed by synchronous fluo rescence spec troscopy.

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Last Update: 2013-04-08