|Table of Contents|

Fabrication of Biosensor Based on Tyrosinase-Graphene Oxide and Its Application(PDF)

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2011年03期
Page:
79-83
Research Field:
化学
Publishing date:

Info

Title:
Fabrication of Biosensor Based on Tyrosinase-Graphene Oxide and Its Application
Author(s):
Wang ShijuanZhu ChaoyunSong WeiLi YulongCao GuoqingNi ChaoWu Xiaoqiong
Department of Applied Chemistry,Nanjing College of Chemical and Technology,Nanjing 210048,China
Keywords:
tyrosinasegraphenecatecholbiosensor
PACS:
TP212.3
DOI:
-
Abstract:
A disposable biosensor has been developed for the determination of catechol in water based on tyrosinase-graphene oxide modified screen printed electrode. Graphene oxide sheets ( GS) were noncovalently-functionalized with 1- pyrenebutanoic acid and succinimidyl ester ( PANHS) . Subsequently,the tyrosinase could covalently bind with PANHSfunctionalized GS by forming amide bond. The as-prepared biocompatible nanocomposite could provide a biocompatible microenvironment to protect the activity of tyrosinase effectively. This disposable biosensor exhibited a fast amperometric response ( less than 9 s) ,and a high sensitivity for monitoring catechol with good repeatability,selectivity and stability. The method showed good linearity in the range from 8. 1 × 10 - 8 mol /L to 2. 2 × 10 - 5 mol /L catechol with a detection limit of 4. 7 × 10 - 8 mol /L. The results showed that the as-prepared disposable biosensor could be used in the rapid and in-situ monitoring of phenolic compounds in water samples.

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Last Update: 2011-09-15