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Construction of Positive-Selection Protein Expression Vectors Based on β-Lactamase(PDF)

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2011年03期
Page:
103-106
Research Field:
生命科学
Publishing date:

Info

Title:
Construction of Positive-Selection Protein Expression Vectors Based on β-Lactamase
Author(s):
Zhu YupengGao JiucaiZhao BiyuShang Guangdong
Jiangsu Key Laboratory for Microbes and Functional Genomics,School of Life Sciences,Nanjing Normal University,Nanjing 210046,China
Keywords:
positive selectioncloning vectorprotein expressionmaltose-binding proteinβ-lactamase
PACS:
Q78
DOI:
-
Abstract:
High efficient gene cloning is the key factor for molecular biology and biochemical research; it is especially important for the manipulations of cloning numerous genes simultaneously,such as the high throughput cloning. Classic cloning method usually involves high background. This paper reports the construction and applications of improved positive selection protein expression vectors of pPAE and pPAE-MBP,with latter the target protein is fused with maltosebinding protein upon protein expression. Experiments showed that both pPAE and pPAE-MBP exhibited 100% cloning efficiency and the target proteins expressed well. pPAE and pPAE-MBP can be used as protein expression vectors in general work especially in the high throughput protein expression research.

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Last Update: 2011-09-15