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Bioconversion of Stevioside by CGT7 Bacteriawith Cyclodextrin Glucanotransferase Activity(PDF)


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Bioconversion of Stevioside by CGT7 Bacteriawith Cyclodextrin Glucanotransferase Activity
Song Tingting1Yin Huihui1Yang Fan2Yan Mang1Chen Yuru12
(1.Jiangsu Engineering and Technology Research Centre for Microbiology Resource,Jiangsu Key Laboratory for Functional Microbes and Genomic,Jiangsu Key Laboratory for Biodiversity and Biotechnology,School of Life Sciences,Nanjing Normal University,Nanjing
cyclodextrin glucanotransferasesteviol glycosideenzymatic modificationglycosyl donorenzyme activity
This work screened the strain CGT7 which produces cyclodextrin glucanotransferase,and studied its bioconversion and modification activities from stevioside into Stv-Glu. The efficient factors were discussed. Experimental results showed that carbon source,nitrogen source,temperature and pH value of culture medium can effect on cyclodextrin glucanotransferase activity of CGT7 observably. Potato starch and soybean cake meal are the appropriate carbon and nitrogen sources with an initial pH value of 8.5 at 30 ℃. The cyclodextrin glucanotransferase activity of CGT7 culture medium reached 544 U/mL under the optimized condition,and the concentration of biocoversion product Stv-Glu was obtained at 0.61 mg/mL when stevioside was introduced as the substrate and cyclodextrin as the glycosyl donor.


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