[1]张卫明,等.三种罗布麻干燥叶片基因组DNA的提取方法[J].南京师大学报(自然科学版),2007,30(01):102-105.
　Zhang Weiming,Wang Manli,Peng Xuemei,et al.Isolation of Genomic DNA From Dried Leaves of Three Kinds of Luobuma[J].Journal of Nanjing Normal University(Natural Science Edition),2007,30(01):102-105.
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三种罗布麻干燥叶片基因组DNA的提取方法()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第30卷
期数:
2007年01期
页码:
102-105
栏目:
生命科学
出版日期:
2007-03-30

文章信息/Info

Title:
Isolation of Genomic DNA From Dried Leaves of Three Kinds of Luobuma
作者:
张卫明1;  2;  王曼丽1;  彭雪梅1;  薛华杰1;  陆长梅1
1. 南京师范大学生命科学学院, 江苏南京210097; 2. 南京野生植物综合利用研究院, 江苏南京210042
Author(s):
Zhang Weiming1; 2; Wang Manli1; Peng Xuemei1; Xue Huajie1;  Lu Changmei1
1.School of Life Science,Nanjing Normal University,Nanjing 210097,China
2. N an jing Institute for C om preh ensive U til ization ofW ild P lan t, N an jing 210042, C h ina
关键词:
罗布麻叶;  DNA提取;  PCR
Keywords:
leav es of Luobum a;  DNA isolation;  PCR
分类号:
S563.7
摘要:
采用Qiagen的植物基因组DNA提取试剂盒并加以改进,首次从不同季节采收、不同干燥方法保存的三种罗布麻(罗布红麻、大叶白麻和白麻)干燥叶片中提取出基因组DNA.提取的基因组TE溶解液的OD260/OD280值多在1.7-1.9范围内,电泳呈现单条带,核基因组的ITS片段、叶绿体基因组的trnL内含子和trnL-F非编码区片段的PCR扩增条带清晰并能进行DNA测序,这表明基因组DNA质量较好,可以满足后续分子生物学实验的要求.本研究为罗布麻等中药材的进一步分子生物学研究提供了必要的技术基础.
Abstract:
Genom ic DNA w as first iso lated from dr ied leaves of Luobum a by the mod ified m e thod of the Q iagen DNeasy plant m in i k it. Resu lts showed tha t by th is me thod genom ic DNA cou ld be iso lated from Luobum a leaves o f three species be ing co llected at d ifferent seasons and be ing preserved by d ifferent m ethods. The OD260 /OD280 ratio s of the iso lated DNA w ere in the range o f 1.7~ 1.9, and only one band presented in agarose ge ls; c lear PCR bands o f ITS rDNA and trnL intron, trnL- F non- cod ing cpDNA cou ld be sequenced. A ll the above revea led tha t the iso lated DNA w as com plete and it cou ld fulfill further dem and fo rm olecu lar b io log ical expe rim en t

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: " 十五"科技攻关基金( 2004BA502B10)、江苏省高新技术研究计划( BG2006318)资助项目.
作者简介: 张卫明( 1957- ) , 教授, 博士生导师, 主要从事野生植物资源的开发和利用研究. E-m ail:botanyzh@ 163. com
通讯联系人: 陆长梅( 1969- ) , 女, 副教授, 主要从事植物学的教学与研究. E-m ail:luchangmei@n jnu. edu. cn
更新日期/Last Update: 2013-05-05