[1]杨瑶,袁生,尚广东,等.烟酸羟基化菌株睾酮丛毛单胞菌JA1抗性质粒的消除[J].南京师大学报(自然科学版),2007,30(02):77-80.
　Yang Yao,Yuan Sheng,Shang Guangdong,et al.Elimination of the Resistance Plasmidin Niconic Acid Hydroxylation Strain Comamonastestosteroni JA1[J].Journal of Nanjing Normal University(Natural Science Edition),2007,30(02):77-80.
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烟酸羟基化菌株睾酮丛毛单胞菌JA1抗性质粒的消除()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第30卷
期数:
2007年02期
页码:
77-80
栏目:
生命科学
出版日期:
2007-06-30

文章信息/Info

Title:
Elimination of the Resistance Plasmidin Niconic Acid Hydroxylation Strain Comamonastestosteroni JA1
作者:
杨瑶;袁生;尚广东;戴亦军;
江苏省生物多样性和生物技术重点实验室, 南京师范大学生命科学学院微生物工程重点实验室, 江苏南京210097
Author(s):
Yang Yao; Yuan Sheng; Shang Guangdong; Dai Yijun
Jiangsu Key Laboratory of Biodiversity and Biotechnology,Key Lab of Microbial Technology in the School of Life Science,Nanjing Normal University,Nanjing 210097,China
关键词:
质粒消除;  烟酸脱氢酶;  Comamonastestosteroni JA1;  抗生素抗性
Keywords:
p lasm id elim ina tion;  nico tin ic ac id dehydrogenase;  Com am onastestoste roni JA1;  antib iotic resistance
分类号:
Q78
摘要:
睾酮丛毛单胞菌JA1可羟基化烟酸产生6-羟基烟酸,并且对多种抗生素具有抗性.碱变性法提取JA1细胞内的质粒,检测到一个约19 kb大小的质粒.用0.002 5%SDS进行质粒消除实验,结果表明,质粒消除后菌株对卡那霉素敏感,但仍对氨苄青霉素、安普霉素和链霉素具有抗性,推测质粒携带卡那霉素的抗性基因.同时,质粒消除后,JA1菌株烟酸脱氢酶酶活性没有变化,推测烟酸脱氢酶的基因并不在质粒上.
Abstract:
Com am onastesto steroni JA1, cata lyzing the hydroxy la tion from n icotin ic ac id to 6- hydroxynico tin icac id, w as found to resist to m any antib io tics. A lkaline lysis ex traction of the JA1 culture reveals a 19 kb p lasm id. 0. 002 5% SDS treatm ent o f the strain e lim inated the ex trachrom osa l plasm id. The m utants lost the kanam ycin res istance ability wh ile keeping the nicotin ic ac id hydroxy lation activ ity.

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备注/Memo

备注/Memo:
基金项目: 江苏省高校自然科学重大基础研究( 06KJA21016 )、江苏省" 六大人才高峰" ( 06- C - 014 )资助项目.
作者简介: 杨 瑶( 1981-) , 女, 博士研究生, 主要从事微生物转化的学习与研究. E-m ail: cranny- yang@ hotm ail. com
通讯联系人: 袁  生( 1956- ) , 教授, 博士生导师, 主要从事微生物转化的教学与研究. E-m ail:yuansheng@ n jnu. edu. cn
更新日期/Last Update: 2013-05-05