[1]吴窈画,邵蔚蓝.产结晶纤维素酶的嗜热菌鉴定及其酶学性质[J].南京师大学报(自然科学版),2007,30(02):84-88.
　Wu Yaohua,Shao Weilan.Identification of Crystalline Cellulose-Degrading Thermophilic Bacterium and the Characterization of Avicelase[J].Journal of Nanjing Normal University(Natural Science Edition),2007,30(02):84-88.
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产结晶纤维素酶的嗜热菌鉴定及其酶学性质()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第30卷
期数:
2007年02期
页码:
84-88
栏目:
生命科学
出版日期:
2007-06-30

文章信息/Info

Title:
Identification of Crystalline Cellulose-Degrading Thermophilic Bacterium and the Characterization of Avicelase
作者:
吴窈画;邵蔚蓝;
南京师范大学微生物工程重点实验室,江苏南京210097
Author(s):
Wu Yaohua; Shao Weilan
The Key Laboratory of Microbiology Engineering,Nanjing Normal University,Nanjing 210097,China
关键词:
亚栖热菌;  16SrDNA;  结晶纤维素酶;  酶活;  酶纯化和性质
Keywords:
M eiotherm us;  16S rDNA;  Avicelase;  enzyme activity;  purification and characterization
分类号:
Q93-3
摘要:
从云南腾冲热海温泉采集的样品中,分离出一株产结晶纤维素酶的嗜热菌TC-1.该菌株呈杆状,革兰氏阴性,生理生化特征分析和16S rDNA序列同源性比对表明它属于亚栖热菌属Meiothermus.结晶纤维素、木聚糖和葡萄糖可以诱导该菌产生结晶纤维素酶,其中结晶纤维素诱导的酶活力最高.对其产生的结晶纤维素酶进行了初步纯化与酶学性质研究,该酶的最适反应温度为70℃,最适pH为6.0,在50℃~70℃和pH5.5~6.5之间酶活力相对稳定.
Abstract:
A rosy-p igmented Gram-negative, thermophilic bacterium which could p roduce avicelase effi ciently,was isolated from Tengchong hot sp rings in Yunnan p rovince. The strain named TC - 1, was iden tified as the genus ofM eiotherm us, based on the results ofmorphological, physio-biochemical characteris tics, and the homological analysis of 16S rDNA sequence. The TC - 1 strain could utilize crystalline cel lulose, carboxymethylcellulose, xylan and glucose. Experimental investigations revealed that crystalline cellulose could induce p lenty of enzyme p roduction. The enzyme was partially purified, itsmaximal activi ty with the substrate of crystalline cellulose was at pH610 and 70℃, respectively. The studies on pH and temperature stability showed that it was stable enough between pH5.5～6.5 at 70℃ for 1 h, and more than 80% of the activity still remained when incubation was p rolonged to 1 h at 70℃.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:江苏省高技术研究项目(BG20050326)资助.
作者简介:吴窈画(1981—) ,女,硕士研究生,主要从事微生物分子生物学的学习与研究. E-mail: wyhsilence2000@126. com
通讯联系人:邵蔚蓝(1958—) ,女,特聘教授,博士生导师,主要从事微生物分子生物学的教学与研究. E-mail: wlshao404@hotmail. com
更新日期/Last Update: 2013-05-05