[1]毛善国,等.番红花及其混淆品的rDNA ITS序列与AS-PCR鉴别[J].南京师大学报(自然科学版),2007,30(02):89-92.
　Mao Shanguo.,Luo Yuming,Shen Jie,et al.Authentication of Crocus sativus L.and its Adulterants by rDNA ITS Sequences and Allele-Specific PCR[J].Journal of Nanjing Normal University(Natural Science Edition),2007,30(02):89-92.
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番红花及其混淆品的rDNA ITS序列与AS-PCR鉴别()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第30卷
期数:
2007年02期
页码:
89-92
栏目:
生命科学
出版日期:
2007-06-30

文章信息/Info

Title:
Authentication of Crocus sativus L.and its Adulterants by rDNA ITS Sequences and Allele-Specific PCR
作者:
毛善国1;  2 ; 罗玉明2 ; 沈　洁1 ; 丁小余1
1. 南京师范大学生命科学学院,江苏南京210097
2. 淮阴师范学院生物系,江苏淮安223300
Author(s):
Mao Shanguo1.2; Luo Yuming2; Shen Jie1; Ding Xiaoyu1
1.School of Life Science,Nanjing Normal University,Nanjing 210097,China
2. Department of Biology, Huaiyin Teachers College, Huaian 223300, China
关键词:
番红花;  rDNAITS区;  混淆品;  位点特异性PCR;  分子鉴别
Keywords:
Crocus sativus L. ;  rDNA ITS region;  adulterant;  allele-specific PCR;  molecular authentica tion
分类号:
S567.239
摘要:
通过对番红花及其混淆品的rDNA ITS区序列进行PCR扩增、测序,并运用Clustal X、Mega 3.0等软件进行序列分析.结果表明番红花rDNA ITS区序列全长650 bp,GC百分比为60.3%,与其混淆品的rDNA ITS区序列存在着显著差异.另外,还设计出了鉴别番红花的位点特异性PCR引物,无需测序即可对番红花及其混淆品进行准确的分子鉴别.
Abstract:
The PCR p roducts of rDNA ITS regions of Crocus sativus L. and its adulterantswere sequenced and analyzed using Clustal X、Mega 3.0 software. The results showed that the full length of rDNA ITS re gions of C. sativus was 650 bp, and the percentage of GC contents was 60.3% , which disp layed the re markable distinction between rDNA ITS regions of C. sativus and its adulterants. In addition, a pair of al lele-specific PCR (AS - PCR) p rimerswere designed to authenticate C. sativus and its adulterants accu rately without sequencing.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:江苏省普通高校高新技术产业发展项目基金( JH02 - 124)资助项目.
作者简介:毛善国(1969—) ,硕士研究生,主要从事植物学的学习与研究. E-mail:msg319@126. com
通讯联系人:丁小余(1965—) ,教授,博士生导师,主要从事药用植物的教学与研究. E-mail: dingxynj@263. net
更新日期/Last Update: 2013-05-05