[1]徐佳宁,许 勤,薛 静,等.小菜蛾泛素基因的克隆及序列分析[J].南京师大学报(自然科学版),2008,31(04):104-107.
　Xu Jianing,Xu Qin,Xue Jing,et al.Cloning and Sequence Analysis of an Ubiquitin Gene of Plutella Xylostella (L.)[J].Journal of Nanjing Normal University(Natural Science Edition),2008,31(04):104-107.
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小菜蛾泛素基因的克隆及序列分析()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第31卷
期数:
2008年04期
页码:
104-107
栏目:
生命科学
出版日期:
2008-12-30

文章信息/Info

Title:
Cloning and Sequence Analysis of an Ubiquitin Gene of Plutella Xylostella (L.)
作者:
徐佳宁1;  许 勤2;  薛 静1;  王 颖1;  程罗根1;  李忠英3
( 1. 南京师范大学生命科学学院, 江苏南京210046)
( 2. 南京师范大学电气与自动化工程学院, 江苏南京210042 )
( 3. 贵州省农业科学院植物保护研究所, 贵州贵阳550006)
Author(s):
Xu Jianing1; Xu Qin2; Xue Jing1; Wang Ying1; Cheng Luogen1; Li Zhongying3
1.School of Life Science,Nanjing Normal University,Nanjing 210046,China
2. School of E lectrical and Autom ation Engin eering, Nan jing Norma lU n ivers ity, Nan jing 210042, China
3. Inst itu te ofP lant Protect ion, Gu izhou Academ y ofAgricu lture S cien ces, Gu iyang 550006, Chin a
关键词:
小菜蛾;  泛素基因;  克隆;  序列分析
Keywords:
P lutella X y lostella( L. );  ub iqu itin g ene;  clon ing;  sequence analysis
分类号:
S433
摘要:
设计了一对简并引物,从小菜蛾Plutella Xylostella(L.)抗溴氰菊酯品系和敏感品系的雌雄成虫基因组中分别克隆了泛素基因的编码区,GenBank登录号分别为EU28778,EU28779,EU28780,EU28781.序列分析表明,该基因的长度为228bp,编码的多肽由76个氨基酸组成.不同品系和不同性别的小菜蛾泛素基因的核苷酸序列、氨基酸序列等均存在差异,其中敏感品系雌雄成虫间差异较大,序列相似性为81.1%;抗性品系雌雄成虫间差异较小,序列相似性为97.8%.推测这些差异可能与小菜蛾抗药性的形成有关. 因而为进一步研究小菜蛾抗药性形成机制和遗传机理奠定了基础.
Abstract:
The c loned ub iqu itin g ene cod ing sequence of P lu tella Xy los tella ( L. ) susceptible stra in and the deltam ethrin - res istance stra in of the adu ltm a le and fem a le, 228 bp in leng th, encodes a pro te in of 76 am ino acids( GenBank Accession No. EU28778, EU28779, EU28780, EU28781). M ultiple sequence a lignment ind ica ted tha t the nucleo tide and am ino ac id sequences both have d ifferent sites among the four sam ples: the susceptib le stra in has larger d ifferences betw een m a le and fema le adu lts, w ith the sequence s im ilar ity o f 81.1%; wh ile the resistant strain has sm a ller d ifferences, sequence sim ilarity 97.8%, thus these differences m ay be related to themo lecu la rm echan ism of drug res istance in P lutella X y lostella, and lay a founda tion for further study o f its fo rm ation and g eneticm echanism s

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 江苏省自然科学基金( BK2006219 )、南京师范大学实践创新训练计划资助项目.
通讯联系人: 程罗根, 博士, 教授, 研究方向: 遗传学. E-mail:chengluogen@ n jnu. edu. cn
更新日期/Last Update: 2013-05-05