[1]吕 宁,等.长型肌球蛋白轻链激酶4Ig结构域的表达及单克隆抗体的制备[J].南京师大学报(自然科学版),2008,31(04):124-128.
　Lü Ning,Zhu Chunhua,Zhang Wencheng,et al.Expression and Monoclonal Antibody Preparation of 4Ig Fragment of Long Myosin Light Chain Kinase[J].Journal of Nanjing Normal University(Natural Science Edition),2008,31(04):124-128.
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长型肌球蛋白轻链激酶4Ig结构域的表达及单克隆抗体的制备()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第31卷
期数:
2008年04期
页码:
124-128
栏目:
生命科学
出版日期:
2008-12-30

文章信息/Info

Title:
Expression and Monoclonal Antibody Preparation of 4Ig Fragment of Long Myosin Light Chain Kinase
作者:
吕 宁1;  2;  朱春花1;  张文程2;  朱敏生2;  陈华群1
( 1. 南京师范大学生命科学学院, 江苏省分子医学生物技术重点实验室, 江苏南京210046 )
( 2. 南京大学模式动物研究所, 江苏南京210061)
Author(s):
Lü Ning1; 2; Zhu Chunhua1; Zhang Wencheng2; Zhu Minsheng2; Chen Huaqun1
1.The Key Laboratory of Molecular and Medical Biotechnology of Jiangsu Province,School of Life Sciences,Nanjing Normal University,Nanjing 210046,China
2. M odelAn im alResearch C enter, Nan jing Un ivers ity, Nan jing 210061, Ch in a
关键词:
MLCK4Ig;  蛋白表达;  单克隆抗体
Keywords:
L- MLCK;  MLCK 4 Ig;  prote in expression;  monoclona l an tibody
分类号:
Q78
摘要:
长型肌球蛋白轻链激酶(long myosin light chain kinase,L-MLCK)及其分子内结构域的功能还不清楚.在研究其功能时,特异性抗体是一基本工具,但目前国际上还没有制备成功特异性较好的抗体.为此,我们先在大肠杆菌E.coliBL21中重组表达了鸡L-MLCK的4Ig蛋白片段(MLCK4Ig),蛋白经纯化后作为抗原免疫小鼠.在小鼠血清中检测到特异性抗体的基础上,建立了2株杂交瘤细胞株,获得抗鸡MLCK4Ig的单克隆抗体.该抗体可以特异与鸡L-MLCK和鸡MLCK4Ig反应.本文并对该抗体的特性及应用进行了讨论. 结果表明, 我们部分解决了L- MLCK的抗体缺乏问题, 为研究L- MLCK及MLCK4 Ig片段的功能提供了重要工具.
Abstract:
The functions of longm yosin light cha in k inase (MLCK) and the m ultip le dom a ins w ith in the m olecule were poorly understood. To our know ledge, there is no specific antibody aga inst L- MLCK so far, wh ich is essen tia l for the study o fL- MLCK. In this report, the 4 Ig fragm ent o f ch icken L- MLCK (MLCK4Ig ) w as expressed by using ofE. co li BL21 system, the pur ified prote in was used as antigen to induce antibody. Spec ific antibody was detected in the sera o f m ouse in jected w ith chickenMLCK4Ig. W e further estab lished two hybr idom a ce ll lines produc ingm onoc lonal an tibody, wh ich specifically reac ted w ith ch ickenMLCK4Ig o r ch icken L- MLCK. The character istic and usefulness o f the ant-i body we re a lso discussed. The resu lts show ed that ourwo rk partially so lved the prob lem o f L- MLCK antibody de fic iency, w hich prov ides an im portant too l fo r the functional investigation of L-M LCK andM LCK4Ig fragm ent

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金( 30470852)、江苏高校自然科学研究计划( 2006104TS J0104)资助项目.
通讯联系人: 陈华群, 副教授, 研究方向: 细胞生物学. E-m a il:chenhuaqun@ n jnu. edu. cn
更新日期/Last Update: 2013-05-05