[1]王玮,金邦荃,陆艳,等.胞外高铁肌红蛋白还原酶电子传递活性位点的研究[J].南京师大学报(自然科学版),2009,32(04):59-64.
　Wang Wei,Jin Bangquan,Lu Yan,et al.Study on the Active Site of MetMbase Electron Transport out of Cell[J].Journal of Nanjing Normal University(Natural Science Edition),2009,32(04):59-64.
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胞外高铁肌红蛋白还原酶电子传递活性位点的研究()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第32卷
期数:
2009年04期
页码:
59-64
栏目:
化学
出版日期:
2009-12-30

文章信息/Info

Title:
Study on the Active Site of MetMbase Electron Transport out of Cell
作者:
王玮;金邦荃;陆艳;刘瑾玲;
南京师范大学食品科学与营养系, 江苏南京210097
Author(s):
Wang Wei; Jin Bangquan; Lu Yan; Liu Jinlin
Department of Food Science and Nutrition,Nanjing Normal University,Nanjing 210097,China
关键词:
高铁肌红蛋白还原酶;  电子传递;  NADH;  NaN3;  N-E thy lm a le im ide;  二价金属离子
Keywords:
M eMt base;  e lec tron transport;  NADH;  NaN 3;   ; N-Ethy lm a le im ide;  d iva lent m eta l ions
分类号:
R341
摘要:
以NADH 为反应体系的启动剂, 研究猪心肌提取液中高铁肌红蛋白还原酶(M eMt base) 活性的表达, 实验结果证实酶活性的表达依赖NADH 启动, 且01 2mM NADH 浓度可充分启动M eMt base活性的表达. 研究还表明, ATP可全程抑制M eMt base呼吸链电子的传递, 抑制酶的活性; NaN3 通过阻断呼吸链第三位点电子传递来抑制酶活性的表达; 而N-E thy lm ale im ide能与M eMt base官能团巯基( - SH )瞬间结合并快速使酶活性丧失; 二价金属离子能干扰活性的表达, Zn 2+ 可促进M eMt base活性的表达; 而Cu 2+ 和M g 2+ 均抑制酶活性的表达, 且Mg 2+ 抑制强度大于Cu 2+ . 金属螯合剂EDTA可掩蔽自由金属离子对M eMt base酶促反应的干扰, 改善酶活性的表达.
Abstract:
M eMt base extrac tion from porc ine cardiac m usc le w as studied on its enzym e b io chem ica l properties. Fo r study ingM eMt base active site, NADH, ATP, NaN3, N-E thy lm a le im ide and d iva lentm e tal ions w ere used as b lo ckers o r agon ists in th is research. The resu lts show ed that the express ion o fM eMt base ac tiv ity depended on NADH starting. The m ost optimum NADH concentration w as 0.2mM so that NADH w as a promo tor forM eMt base. In the reaction system, M eMt base activ ity w as inhib ited by the h igher ATP leve ,l because ATP cou ld block e lec tron transport in resp ira tory cha in. A t the third site M eMt base activ ity w as blocked by NaN3. M eMt base was inac tive through N-E thy lm a le im ide bond ing the sulfhydry l g roup( - SH ) in enzym e prote in structure at them om ent. In addition, M eMt base ac tiv ityw as inter fered by som e div alent m eta l ions, such as Zn 2+ , Cu 2+ andM g 2+ . M eMt base activ ity w as increased by Zn 2+ , wh ile it was decreased by Cu 2+ andMg 2+ . The inhib ition ofM g 2+ w as mo re than that of Cu 2+ . The freem e tal ions could be blocked by EDTA as a m eta l chelato r. When EDTA w as added in reaction sy stem, M eMt base activ ity w as expressed enough.

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备注/Memo

备注/Memo:
基金项目国家“863”科技攻关项目(2002AA242031-5);江苏省六大人才高峰项目(184080H10202);南京师范大学研究生优秀学位论文培育计划项目(181200000226)资助项目
通讯联系人: 金邦荃, 博士, 教授, 研究方向: 食品肉品学. E-m ail: jinb angquan@ n jnu. edu. cn
更新日期/Last Update: 2013-04-23