[1]马鹏娟,刘中华,戴亦军,等.嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia)R551-3四环素抗性消除的研究[J].南京师大学报(自然科学版),2010,33(01):84-89.
　Ma Pengjuan,Liu Zhonghua,Dai Yijun,et al.Study on Elimination of the Tetracycline Resistance in Stenotrophomonas maltophilia R551-3[J].Journal of Nanjing Normal University(Natural Science Edition),2010,33(01):84-89.
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嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia)R551-3四环素抗性消除的研究()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第33卷
期数:
2010年01期
页码:
84-89
栏目:
生命科学
出版日期:
2010-03-20

文章信息/Info

Title:
Study on Elimination of the Tetracycline Resistance in Stenotrophomonas maltophilia R551-3
作者:
马鹏娟;刘中华;戴亦军;袁生;
江苏省微生物资源产业化工程技术研究中心, 江苏省生物多样性和生物技术重点实验室, 南京师范大学生命科学学院, 江苏南京210046
Author(s):
Ma Pengjuan; Liu Zhonghua; Dai Yijun; Yuan Sheng
Jiangsu Provincial Engineering and Technology Research Center for Microbiology Resource Industrialization,Jiangsu Key Laboratory of Biodiversity and Biotechnology,School of Life Science,Nanjing Normal University,Nanjing 210046,China
关键词:
嗜麦芽寡养单胞菌;  吡虫啉;  电穿孔;  抗性消除;  四环素
Keywords:
Steno trop hom onasmaltoph ilia;  im idaclopr id;  e lectroporation;  res istance e lim ination;  tetracyc line
分类号:
X172
摘要:
嗜麦芽寡养单胞菌可共代谢降解烟碱类农药吡虫啉,并且对多种抗生素具有抗性.本文通过电穿孔法消除了该菌的四环素抗性,获得了四环素敏感型菌株R551-3 Tcr-,可以用作遗传转化系统的受体菌,能被转化吸收遗传载体质粒pJB866H::ndhSL,构成一对很好的嗜麦芽寡养单胞菌遗传转化系统.同时HPLC结果表明所获得的四环素敏感型菌株羟基化吡虫啉的活性并未发生改变,为进一步开展敲除和回补共代谢途径关键酶的研究工作奠定了基础.
Abstract:
S teno trophom onas maltophilia can deg rade neon icotino id insectic ide im idacloprid through co-me tabo lism and exhibits m ultiple antib io tic resistance. Through e lectroporation we obta ined a R551-3 mu tant w hich lost tetracy cline res istance. Besides, the te tracyc line resistant plasm id pJB866H: : ndhSL w as successfu lly transform ed into the R551-3T crstra in. Therefore, the R551-3 Tcr- stra in can be used as a rec ipien t to construct a Steno trophom onasm altop hilia gene tic transform ation sy stem. The tetracycline resistance lostm utan t keeps the ability o f hydroxy lating im idac lopr id. Based on this mutant, the fo llow ing gene knockou t and recovery exper im ents can be carried out.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金( 30970040)、江苏省高校自然科学基金( 09K JB180003 )、江苏省高校自然科学重大基础研究( 06KJA21016) . 通讯联系人: 袁生, 教授, 博士生导师, 研究方向: 微生物转化. E-mail:yuansheng@ njnu. edu. cn
更新日期/Last Update: 2013-04-08