[1]朱 云,金邦荃,龚 兰,等.猪背最长肌PPARγ2基因Bsr1位点多态性与肉质关系的研究[J].南京师大学报(自然科学版),2013,36(03):81-86.
　Zhu Yun,Jin Bangquan,Gong Lan,et al.Association of PPARγ2 Bsr1 Locus Polymorphism in Longissimus Dorsi With Meat Quality Traits in Swine[J].Journal of Nanjing Normal University(Natural Science Edition),2013,36(03):81-86.
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猪背最长肌PPARγ2基因Bsr1位点多态性与肉质关系的研究()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第36卷
期数:
2013年03期
页码:
81-86
栏目:
生命科学
出版日期:
2013-09-30

文章信息/Info

Title:
Association of PPARγ2 Bsr1 Locus Polymorphism in Longissimus Dorsi With Meat Quality Traits in Swine
作者:
朱 云1; 金邦荃1; 龚 兰1; 章熙霞2; 袁永刚2; 周 鑫1; 徐小波3
(1.南京师范大学食品科学与营养系,江苏 南京 210023) (2.南京市畜牧家禽科学研究所,江苏 南京 210036) (3.江苏省农业科学院畜牧研究所,江苏 南京 210014)
Author(s):
Zhu Yun1; Jin Bangquan1; Gong Lan1; Zhang Xixia2; Yuan Yonggang2; Zhou Xin1; Xu Xiaobo3
(1.Department of Food Science and Nutrition,Nanjing Normol University,Nanjing 210023,China) (2.Institute of Animal Husbandry and Poultry Science of Nanjing,Nanjing 210036,China) (3.Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
关键词:
PPARγ2; Bsr1位点; 多态性; 肉质性状; 猪
Keywords:
PPARγ2; Bsr1 locus; polymorphism; meat quality traits; swine
分类号:
S813.1
摘要:
采用RT-PCR、PCR-RFLP及测序技术,对山猪等背最长肌(LD)的品质及PPARγ2基因表达量和第一启动子Bsr1位点多态性进行研究.结果表明,LD上PPARγ2基因表达量较低,且与肌内脂肪(IMF)呈弱相关(R=0.22).对PPARγ2 Bsr1位点多态性研究发现,该位点可产生G/A突变,形成b、Bb和B型3种基因型.山猪LD PPARγ2 Bsr1位点包含3种基因型,二元杂交后b型比例下降,三元杂交会出现b型缺失.同时发现,长白和大约克LD b型不表达,长白猪仅有B型,而申农1号除B型外Bb型高表达,提示瘦肉猪定向选育过程中可能会使b型逐渐丢失或表达关闭,从而不利于脂肪细胞的增生.以PPARγ2基因型为主效因素与肉质性状拟合建立GLM模型,不同基因型间猪LD剪切力和IMF存在显著差异(P<0.05).b型的高表达有利于脂肪细胞分化和增生,故可维系较高的IMF和较好的肌肉嫩度,提示其可作为优质肉的目标基因.
Abstract:
Longissimus dorsi(LD)in swine(7 breeds),was used to study PPARγ2 gene and its Bsr1 locus(located in PPARγ2 promoter 1)polymorphism with meat quality traits via RT-PCR,PCR-RFLP and sequencing technology.The result showed that the expression of PPARγ2 was low in swine LD.Its correlation with intramuscular fat was weak(R=0.22).It was also found that the base G in wild type b(CCAGT)was replaced by A.b type of Bsr1 locus would be changed to B type(CCAAT)thus it formed b,Bb and B type.Shanzhu LD had 3 genotypes,while its crossbreds had a low frequency of b type or even no b type.We also noticed that there was a high frequency of Bb type expressed in Shennong 1 and no b type in Landrace and Yorkshire.GLM model was used to analyze the association of genotypes(as a major factor)with meat quality traits.It was indicated that genotypes of PPARγ2 Bsr1 locus were correlated with the shear force of meat and intramuscular fat,significantly(P<0.05).This suggested that the high expression of b type contributed to adipose differentiation,deposition and appropriate for meat tenderness.It also means that PPARγ2 gene can be the meat target gene.

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备注/Memo

备注/Memo:
收稿日期:2013-05-07.
基金项目:江苏省农业科技创新基金(CX(11)1301).
通讯联系人:金邦荃,教授,研究方向:食品肉品学.E-mail:jinbangquan@njnu.edu.cn
更新日期/Last Update: 2013-09-30